Example sentences for: uracil

How can you use “uracil” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • A screen of a HeLa cell library yielded 56 colonies that tested positive in the two hybrid assays (growth on plates lacking histidine or uracil, lack of growth on plates containing 5-fluoroorotic acid; and induction of lacZ resulting in blue color when assayed with X-gal).

  • SC medium is 1.7 g/l yeast nitrogen base without amino acids and ammonium sulfate, 5 g/l ammonium sulfate, 20 g/l glucose, 20 mg/l uracil, 20 mg/l adenine, 80 mg/l L-leucine, 20 mg/l L-histidine, 40 mg/l L-tryptophan, 20 mg/l L-methionine, and 30 mg/l L-lysine.

  • Then 500 cells were plated onto selective and non-selective (EMM+uracil) plates to determine the fraction of cells containing plasmid.

  • In our construct, we deleted this adaptor to prevent use of the start codon for translation; that would result in nonexpression of the functional URA3 gene and, therefore, an inability to grow in the absence of uracil.

  • Cells were grown at 30°C in EMM [ 22] supplemented with uracil and leucine (150 mg each per liter) when not under selection or leucine (150 mg per liter) when under selection for uracil prototrophy.


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