Words similar to uranyl
Example sentences for: uranyl
How can you use “uranyl” in a sentence? Here are some example sentences to help you improve your vocabulary:
1% gelatin, 1% normal goat serum, 0.3% Triton-X-100 in TBS), placed in primary antibody (1:10 diluted in blocking buffer for all antibodies used) for 2 hours at room temperature, rinsed 3 times for 5 min each in TBS, placed in gold-conjugated secondary antibodies (10 nm gold anti-mouse and 5 nm gold anti-rabbit) for 1 hour, rinsed 3 times in TBS, fixed in 2% glutaraldehyde, rinsed 3 times in TBS, rinsed in water, stained with Reynold=s lead citrate followed by aqueous 2% uranyl acetate, dried on filter paper and viewed using a Philips CM10 transmission electron microscope.
The samples were post-fixed with 1% glutaraldehyde and stained with uranyl acetate and lead citrate.
After staining with osmium tetroxide and uranyl acetate, NPCs in wild-type/parental cells were represented by electron dense structures spanning the pore (Figure 4).
For electron microscopy imaging, ultra-thin sections were stained with uranyl acetate and lead citrate (5 minutes each) and viewed under a Philips CM10 transmission electron microscope.
Earlier studies have shown that DAP, in DNA fragments, can widen the minor groove, as detected by reactivity towards uranyl nitrate or susceptibility to Dnase cleavage [ 29 ] , and can relieve compressions in the minor groove associated with A-tracts [ 30 ] . In contrast, NMR and molecular modeling studies of an oligonucleotide duplex containing MeC have demonstrated that, while this duplex still has a B-DNA conformation, there are differences in the structural parameters and thermal stability relative to the unmodified duplex [ 31 ] . An earlier study also found, in two closely related octanucleotide duplexes, that although the methylated duplexes retained their B-DNA conformation, different structural and thermal stability effects were seen [ 32 ] . Although these studies have been carried out with DNA-DNA and not DNA-RNA duplexes, it is possible that MeC substitution may have different structural consequences than DAP substitution.
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