Words similar to ura
Example sentences for: ura
How can you use “ura” in a sentence? Here are some example sentences to help you improve your vocabulary:
The TAR vector pARS-SV211-URA was constructed by insertion of the S. cerevisiae URA3 gene between the targeting hooks of pVC-SV211 [ 7 ] and cloning of ARSH4 into the polylinker.
The yeast strains used are Y312 ( MATα ura3 leu2 his3 Gal +), Y1284 (
The highly transformable S. cerevisiae strain VL6-48N ( MATalpha, his3-Δ200, trp1-Δ1 , ura3-Δ1 , lys2 , ade2-101, met14 ), which has HIS3 and URA3 deletions, was used for transformation.
HL4000AA) into strain PJ69-4A ( MAT a, trp 1-190 leu2-3, 112 ura3-52 his3-200 gal4 gal80 LYS2::GAL1-HIS3 GAL2-ADE2 met2::GAL7-lacZ) [ 41 ] . Strong expression of full length GALDB-TOP1 in PJ69-4A was verified by Western blotting with antibodies to both TOP1 and GALDB (not shown).
The resulting YAC does not express URA3 and, therefore, the clone can be selected by medium containg 5-FOA.
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