Words similar to ura
Example sentences for: ura
How can you use “ura” in a sentence? Here are some example sentences to help you improve your vocabulary:
The targeting hooks were placed in the promoter region of URA3 as shown in Figure 2. Genomic DNA and the linearized TAR cloning vector were combined with yeast spheroplasts and the His +Ura -transformants were selected on medium containing 5-FOA to identify clones with genomic DNA inserts.
Deletion of MIG1 was achieved by transforming strain yHZ416 with the Hind III digest of pJN22 (for migl-Δ2::LEU2) or pJN41 (for mig1-Δ2::URA3) [ 4], and selecting for Leu +or Ura +transformants, respectively.
In this case, the distance between the TATA element and the transcription initiation site is not changed, and URA3 is expressed normally, rendering the clone sensitive to 5-FOA.
To determine if Tab +phenotypes were due to single mutations, cdc15Δ tab strains were crossed to cdc15Δ [pMET3-cdc15-2, URA3] and the resulting diploids were sporulated and dissected.
ADH1-URA3 cassette included PCR amplification of the ADH1 promoter and the URA3 gene from the pBL94 plasmid using two pairs of primers, ADH-F plus ADH-R and URA-ARS-F plus URA-ARS-R (Table 2).