Words similar to ura
Example sentences for: ura
How can you use “ura” in a sentence? Here are some example sentences to help you improve your vocabulary:
As a first step toward understanding the mechanism of replication enhancement, we have generated consecutive ~50-bp deletions throughout the ura4 replication enhancer (Fig.
Diploid strain BY71 (Table 1), created to be heterozygous for MAT , ADE2 , HIS3 , LEU2 , LYS2 , MET15 , TRP1 , URA3 and HNT2 , was allowed to sporulate and was then dissected.
As in the first approach, the TAR vector contained a yeast centromere ( CEN6 ), a yeast ARS element ( ARSH4 ), a positive-selectable marker ( HIS3 ), and a negative-selectable marker ( URA3 ). In this approach, however, the targeting hooks are placed between the promoter and the open reading frame of the URA3 gene.
The TAR vector pARS-SV211-URA was constructed by insertion of the S. cerevisiae URA3 gene between the targeting hooks of pVC-SV211 [ 7 ] and cloning of ARSH4 into the polylinker.
The targeting hooks were placed in the promoter region of URA3 as shown in Figure 2. Genomic DNA and the linearized TAR cloning vector were combined with yeast spheroplasts and the His +Ura -transformants were selected on medium containing 5-FOA to identify clones with genomic DNA inserts.
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