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Example sentences for: uppsala

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The supernatant was incubated with glutathione sepharose 4B beads (Amersham Pharmacia Biotech AB, Uppsala, Sweden) and washed as previously described.
The van der Waals, bond, angle, dihedral, and improper dihedral parameters for all the ligands were adopted from the Hetero-compound Information Centre-Uppsala HIC-Up http://xray.bmc.uu.se/hicup [ 55 ] . The water molecule under the flaps present in all complexes was included in all steps throughout this study except for 1hvr, where the structural water was removed in the preparation of the protease to be docked with the ligand (the ligand of 1hvr, which is a urea-based inhibitor, does not bind a water molecule in this position).
Gel elecrophoresis was run in 1 X TBE buffer at 200 V with the circulator set at 4°C (using a Penquin Water-cooled Dual-Gel Electrophoresis system attached to a thermostatically controlled refrigerated circulator, Amersham & Pharmacia, Uppsala, Sweden).
Following chromatographic separation of unincorporated 35SO 4 2-using PD-10 columns (Pharmacia, Uppsala, Sweden), the levels of radiolabeled GAGs released onto the culture media or recovered by alkaline extraction were quantitated by scintillation counting [ 24 ] .
Heparinized venous blood from RA patients, SLE patients and healthy control individuals was collected, and peripheral blood mononuclear cells were freshly isolated by Ficoll-Hypaque (Amersham Pharmacia Biotech, Uppsala, Sweden).