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Example sentences for: uppsala
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Briefly, the hybridization medium contained 3 × SSC, 1.5 μg/μl poly(A) DNA (Pharmacia Biotech, Uppsala), and 0.5% SDS.
Gel elecrophoresis was run in 1 X TBE buffer at 200 V with the circulator set at 4°C (using a Penquin Water-cooled Dual-Gel Electrophoresis system attached to a thermostatically controlled refrigerated circulator, Amersham & Pharmacia, Uppsala, Sweden).
Following chromatographic separation of unincorporated 35SO 4 2-using PD-10 columns (Pharmacia, Uppsala, Sweden), the levels of radiolabeled GAGs released onto the culture media or recovered by alkaline extraction were quantitated by scintillation counting [ 24 ] .
All patients were ventilated using a Servo 300 Siemens ventilator (Siemens Elema, Uppsala, Sweden) using the pressure-regulated volume control mode with a tidal volume of 8-10 ml/kg (based on ideal body weight), frequency of 12 breaths/min, fraction of inspired oxygen of 1.0, and inspiratory:expiratory ratio of 1:2.
The eggs were extracted in Celis lysis buffer (see [ 46 ] ) containing 2% (v:v) NP-40, 9.8 M urea, 100 mM dithiothreitol (DTT), 2% ampholines (pH 3.5-10), and protease inhibitors for 30 min at room temperature, according to the protocol described in [ 47 ] . Isoelectric focusing of the egg extracts (IEF) was performed using the Mini-PROTEAN II tube cell apparatus according to the manufacturer's protocol (Bio-Rad, Richmond, CA) with ampholines of pH 3.5-5 (30%), 3.5-10 (40%), 5-7 (20%), and 7-9 (10%), (Pharmacia Biotech, Uppsala, Sweden).
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