Words similar to uppsala

Example sentences for: uppsala

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All patients were ventilated using a Servo 300 Siemens ventilator (Siemens Elema, Uppsala, Sweden) using the pressure-regulated volume control mode with a tidal volume of 8-10 ml/kg (based on ideal body weight), frequency of 12 breaths/min, fraction of inspired oxygen of 1.0, and inspiratory:expiratory ratio of 1:2.
The eggs were extracted in Celis lysis buffer (see [ 46 ] ) containing 2% (v:v) NP-40, 9.8 M urea, 100 mM dithiothreitol (DTT), 2% ampholines (pH 3.5-10), and protease inhibitors for 30 min at room temperature, according to the protocol described in [ 47 ] . Isoelectric focusing of the egg extracts (IEF) was performed using the Mini-PROTEAN II tube cell apparatus according to the manufacturer's protocol (Bio-Rad, Richmond, CA) with ampholines of pH 3.5-5 (30%), 3.5-10 (40%), 5-7 (20%), and 7-9 (10%), (Pharmacia Biotech, Uppsala, Sweden).
Gel elecrophoresis was run in 1 X TBE buffer at 200 V with the circulator set at 4°C (using a Penquin Water-cooled Dual-Gel Electrophoresis system attached to a thermostatically controlled refrigerated circulator, Amersham & Pharmacia, Uppsala, Sweden).
The supernatant was incubated with glutathione sepharose 4B beads (Amersham Pharmacia Biotech AB, Uppsala, Sweden) and washed as previously described.
Heparinized venous blood from RA patients, SLE patients and healthy control individuals was collected, and peripheral blood mononuclear cells were freshly isolated by Ficoll-Hypaque (Amersham Pharmacia Biotech, Uppsala, Sweden).