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Example sentences for: uppsala

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Heparinized venous blood from RA patients, SLE patients and healthy control individuals was collected, and peripheral blood mononuclear cells were freshly isolated by Ficoll-Hypaque (Amersham Pharmacia Biotech, Uppsala, Sweden).
All patients were ventilated using a Servo 300 Siemens ventilator (Siemens Elema, Uppsala, Sweden) using the pressure-regulated volume control mode with a tidal volume of 8-10 ml/kg (based on ideal body weight), frequency of 12 breaths/min, fraction of inspired oxygen of 1.0, and inspiratory:expiratory ratio of 1:2.
The supernatant was incubated with glutathione sepharose 4B beads (Amersham Pharmacia Biotech AB, Uppsala, Sweden) and washed as previously described.
The eggs were extracted in Celis lysis buffer (see [ 46 ] ) containing 2% (v:v) NP-40, 9.8 M urea, 100 mM dithiothreitol (DTT), 2% ampholines (pH 3.5-10), and protease inhibitors for 30 min at room temperature, according to the protocol described in [ 47 ] . Isoelectric focusing of the egg extracts (IEF) was performed using the Mini-PROTEAN II tube cell apparatus according to the manufacturer's protocol (Bio-Rad, Richmond, CA) with ampholines of pH 3.5-5 (30%), 3.5-10 (40%), 5-7 (20%), and 7-9 (10%), (Pharmacia Biotech, Uppsala, Sweden).
Briefly, the hybridization medium contained 3 × SSC, 1.5 μg/μl poly(A) DNA (Pharmacia Biotech, Uppsala), and 0.5% SDS.