Example sentences for: tx

How can you use “tx” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The first round combined reverse transcription using oligo-dT (15)-T7 (5'-AAACGACGGCCAGTGAATTGTAATACGACTCACTATAGGCGC-3') primer and template-switch primer (5'-AAGCAGTGGTATCAACGCAGAGTACGCGGG-3') (Clontech, Palo Alto, CA) catalyzed by the Superscript-11 reverse transcriptase (Gibco-BRL) and Advantage cDNA polymerase (Clontech) for single- and double-strand cDNA synthesis respectively, with in vitro transcription (Megascript kit, Ambion, Austin, TX).

  • Cultured MDA-MB 231 cells (American Type Culture Collection, Rockville, MD) were harvested, rinsed then suspended in serum-free M3D base culture medium (INCELL Corporation, LLC, San Antonio, TX).

  • Ribonuclease protection assays were performed using the RPA III Ribonuclease Protection Assay Kit from Ambion (Austin, TX) according to the manufacturer's specifications.

  • Each PCR product was ligated to adapters that contained an SP6 promoter sequence (SP6 adapter) in one reaction or to adapters with a T7 promoter sequence (T7 adapter) in another reaction according to the Lig'nScribe™ (Ambion, Austin, TX) protocol.

  • Following three PB washes, tissue was then incubated in biotinylated goat-anti-rabbit-IgG (1:200, Vector Labs, Burlingame, CA) in 0.1% Tx100 in PB overnight at 4°C.


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