Words similar to tx
Example sentences for: tx
How can you use “tx” in a sentence? Here are some example sentences to help you improve your vocabulary:
A BRCA1 ribonuclease protection probe was made using an In Vitro Transcription Kit (Ambion, Inc, Austin, TX, USA).
Single-stranded cDNA was synthesized from total RNA using MMLV reverse transcriptase (Retroscript RT kit; Ambion, Inc., Austin, TX, USA).
The probe was hybridized to a poly(A) +RNA Northern blot of mouse tissues (Origene Technologies, Rockville, MD) in ULTRAhyb hybridization buffer (Ambion, Austin, TX) overnight at 42°C and washed according to the manufacturer's instructions.
These zones of inhibition were much larger when pT2TFXK was added to F2/5 and unchanged with the addition of pT2TX3K.
The first round combined reverse transcription using oligo-dT (15)-T7 (5'-AAACGACGGCCAGTGAATTGTAATACGACTCACTATAGGCGC-3') primer and template-switch primer (5'-AAGCAGTGGTATCAACGCAGAGTACGCGGG-3') (Clontech, Palo Alto, CA) catalyzed by the Superscript-11 reverse transcriptase (Gibco-BRL) and Advantage cDNA polymerase (Clontech) for single- and double-strand cDNA synthesis respectively, with in vitro transcription (Megascript kit, Ambion, Austin, TX).