Words similar to tx
Example sentences for: tx
How can you use “tx” in a sentence? Here are some example sentences to help you improve your vocabulary:
The first round combined reverse transcription using oligo-dT (15)-T7 (5'-AAACGACGGCCAGTGAATTGTAATACGACTCACTATAGGCGC-3') primer and template-switch primer (5'-AAGCAGTGGTATCAACGCAGAGTACGCGGG-3') (Clontech, Palo Alto, CA) catalyzed by the Superscript-11 reverse transcriptase (Gibco-BRL) and Advantage cDNA polymerase (Clontech) for single- and double-strand cDNA synthesis respectively, with in vitro transcription (Megascript kit, Ambion, Austin, TX).
Cultured MDA-MB 231 cells (American Type Culture Collection, Rockville, MD) were harvested, rinsed then suspended in serum-free M3D base culture medium (INCELL Corporation, LLC, San Antonio, TX).
Ribonuclease protection assays were performed using the RPA III Ribonuclease Protection Assay Kit from Ambion (Austin, TX) according to the manufacturer's specifications.
Each PCR product was ligated to adapters that contained an SP6 promoter sequence (SP6 adapter) in one reaction or to adapters with a T7 promoter sequence (T7 adapter) in another reaction according to the Lig'nScribe™ (Ambion, Austin, TX) protocol.
Following three PB washes, tissue was then incubated in biotinylated goat-anti-rabbit-IgG (1:200, Vector Labs, Burlingame, CA) in 0.1% Tx100 in PB overnight at 4°C.
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