Words similar to two-hybrid
Example sentences for: two-hybrid
How can you use “two-hybrid” in a sentence? Here are some example sentences to help you improve your vocabulary:
Finally, mutations within the β-box (especially M2405A, L2406A) disrupted ERβ interactions with N-CoR in mammalian two-hybrid assays, but did not affect TRβ interactions (Fig.
1and materials and methods) was tested using the yeast two-hybrid system and found to interact with the extracellular domain of proHB-EGF (Additional file 1).
1) can substitute in function for Boi1 and Boi2 [ 1 ] . In addition, Boi1-PH contains the region of Boi1 that displays the two-hybrid interaction with Cdc42, raising the possibility that the PH domain, itself, mediates or regulates the association with Cdc42 [ 1 ] .
The functions of Boi1/Boi2 appear to be linked to those of Cdc42 and Rho3, which are Rho-type GTPases that are important for polarized cell growth [ 4 5 6 7 8 9 ] . Boi1 and Boi2 display two-hybrid interactions with both wild-type and mutationally activated Cdc42 but not with a mutant version of Cdc42 that is predicted to be impaired in the ability to bind nucleotide, suggesting that Boi1 and Boi2 may associate with the GTP-bound ("active") form of Cdc42 [ 1 ] . Further evidence that some function of Boi1 is linked to that of Cdc42 is that overexpression of Boi1 inhibits bud emergence and that this inhibition can be suppressed by overexpression of Cdc42 [ 1 2 ] . RHO3 can serve as a multicopy suppressor of the lethality caused by deletion of BOI1 and BOI2 [ 1 2 ] . These findings are consistent with the possibilities that Boi1 and Boi2 are targets of Cdc42 that promote cell growth in a manner that is regulated by Rho3.
Identification of TOP1-interacting proteins using two-hybrid assays
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