Words similar to two-hybrid
Example sentences for: two-hybrid
How can you use “two-hybrid” in a sentence? Here are some example sentences to help you improve your vocabulary:
The B em 1 - i nteracting proteins Boi1 and Boi2 were identified from screens for proteins that display two-hybrid interaction with the b ud- em ergence protein Bem1 [ 1 2 ] , a protein that is important for the initiation of budding and that contains two SH3 domains.
Short cytoplasmic domains (5-14 amino acids) are found at both the amino and carboxyl termini of CD9 [ 14 ] . As reported previously, we used a yeast two-hybrid system and observed interaction between the second extracellular domain of MkCD9 and the extracellular domain of proHB-EGF [ 9 ] . However, despite repeated attempts, using the yeast two-hybrid system, we were unable to show interaction between the amino terminal cytoplasmic domain of monkey CD9 and the cytoplasmic domain of proHB-EGF; these results may reflect the limitation of the yeast two-hybrid system as the insert sizes cloned into the vectors may be too small resulting in Gal4 fusion proteins whose "test subunits" are masked by the Gal4 subunit.
Yeast two-hybrid screen and tests
Although yeast two-hybrid interactions can not be easily quantified, the very high level of β-Galactosidase expressed by the pBD-LeMAF1/pAD-LeFPP stain suggests a comparably strong interaction in the yeast two-hybrid screen.
Interestingly, SNIP1 binds to Az and HsN3 in the yeast two-hybrid system, in addition to its ability to bind Smad1 (Fig.
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