Words similar to two-hybrid
Example sentences for: two-hybrid
How can you use “two-hybrid” in a sentence? Here are some example sentences to help you improve your vocabulary:
A series of papers has explored properties of random sequences, including random peptides, fused to DNA binding domains in the context of transcriptional activation assays similar to those used in the two-hybrid system.
While two-hybrid screens efficiently identify fusion proteins that are able to interact, the biological significance of the interaction for native proteins acting in vivo generally requires verification, because the technique is susceptible to a high rate of false positives [ 8 ] . To assess the possible contribution of false-positive protein-protein interactions to the combined interaction dataset, we analyzed the connectivity of each protein and found that a small fraction of proteins had a very high number of interactions (highlighted in red, Fig.
3and materials and methods) was tested using the yeast two-hybrid system and found to interact with the extracellular domain of proHB-EGF (Table 1).
Short cytoplasmic domains (5-14 amino acids) are found at both the amino and carboxyl termini of CD9 [ 14 ] . As reported previously, we used a yeast two-hybrid system and observed interaction between the second extracellular domain of MkCD9 and the extracellular domain of proHB-EGF [ 9 ] . However, despite repeated attempts, using the yeast two-hybrid system, we were unable to show interaction between the amino terminal cytoplasmic domain of monkey CD9 and the cytoplasmic domain of proHB-EGF; these results may reflect the limitation of the yeast two-hybrid system as the insert sizes cloned into the vectors may be too small resulting in Gal4 fusion proteins whose "test subunits" are masked by the Gal4 subunit.
Interestingly, both ubiquitin and Az were isolated from the yeast two-hybrid system as Smad1 interactors.
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