Example sentences for: tsp-
How can you use “tsp-” in a sentence? Here are some example sentences to help you improve your vocabulary:
The expression of the proteins TSP-1 and TYRP-1 were found to correlate with the nonmetastatic phenotype, whereas OPN was significantly elevated in the metastatic counterpart.
The sequences of the PCR primer pairs used for each gene are as follows: TYRP-1, 5'-GATGGCAGAGATGATCGGGA-3' and 5'-AGAAATTGCCGTTGCAGTGAC-3' ; TSP-1, 5'-CAGCAGCCGCTTTTATGT-3' and 5'-CCGAGTATCCCT-GAGCCCTC-3' ; OPN, 5'-TTGCAGCCTTCTCAGCCAA-3' and 5'-GGAGGCAAAAGCAAATCACTG-3' ; and GAPDH, 5'-CCACCCATGGCAAATTCC-3' and 5'-GGGATTTCCATTGATGACA-3'.
FFPE = formalin-fixed, paraffin-embedded; H&E = hematoxylin and eosin; IHC = immunohistochemistry; LCM = laser capture microdissection; OPN = osteopontin; PBS = phosphate-buffered saline; PCR = polymerase chain reaction; qPCR = real-time, quantitative PCR; RT = reverse transcriptase; TSP-1 = thrombospondin-1; TYRP-1 = tyrosinase-related protein-1.
Primer sets for OPN, TSP-1, TYRP-1 and glyceraldehyde-3-phosphate-dehydrogenase (GAPDH, the housekeeping gene) were designed using Primer Express Version 1.5 software (Applied Biosystems, Foster City, CA, USA).
TYRP-1 expression was higher than that of TSP-1 but lower than that of OPN (10-fold to 50-fold).