Words similar to trypsin-

Example sentences for: trypsin-

How can you use “trypsin-” in a sentence? Here are some example sentences to help you improve your vocabulary:

HEL cells were dislodged from the flask with a trypsin-EDTA solution, washed with DEPC-treated PBS, and then resuspended in 6 ml of ice-cold resuspension buffer [10 mM Tris-HCl (pH 7.5), 10 mM KC1, 5 mM MgCl 2 ]. Six ml of hot (70°C) lysis buffer [20 mM Tris-HCl (pH 7.4), 0.4 M NaCI, 40 mM EDTA, 1% β-mercaptoethanol, 1% SDS] and 1 ml of citrate-buffered phenol (pH 4.0) were added to each cell suspension.
After the cells reached approximately 80% confluence (5 to 9 days), they were removed from flasks with 0.025% trypsin-0.
Subconfluent fibroblasts were trypsinized (trypsin-EDTA; 0.05% trypsin, 0.53 mM EDTA-4 Na) and used for collagen gel culture.
5 μl of 0.25% trypsin-EDTA (Gibco-BRL) was used as a positive control for the casein zymogram.
To isolate rat neonatal microglia, cerebral cortices of 1-2 day-old Sprague-Dawley rats purchased from Harlan (Indianapolis, IN) were surgically removed and placed in cold DMEM + 10% FBS +120 U/ml P and 12 μg/ml S, the meninges carefully removed, and brain tissue minced and dissociated with trypsin-EDTA at 36°C for 3-5 min.