Example sentences for: trypan

How can you use “trypan” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • In an attempt to determine if increased survival of the antisense cells was do to increased proliferation of the antisense cells or decreased death rate, the number of trypan blue positive, non-viable were examined after 14 days without estrogen.

  • RPMI-1640 medium without phenol red, cell culture freezing medium containing DMSO, fetal bovine serum (FBS), penicillin, streptomycin, 7.5% bovine serum albumin (BSA), phosphate buffer saline (PBS, pH = 7.4), and trypan blue were purchased from Life Technologies (Grand Island, NY).

  • Tissues were then pinned flat on wax blocks and covered with trypan blue (0.

  • Cell viability was quantified by trypan blue exclusion analysis at the beginning of every experiment to ensure that cell cultures used in the experiments were healthy (alive cells >95%).

  • Placental tissue was digested and trophoblast cells isolated and cultured as described previously [ 15 36 ] . Briefly, the cells collected after digestion were pooled, washed twice in DMEM containing 25 mM HEPES and 4500 mg/L glucose (DMEM-HG), diluted to 10 ml in DMEM-HG supplemented with heat inactivated 5% fetal bovine serum (FBS) and antibiotics (50 μg/ml gentamycin, 100 U/ml penicillin, and 100 μg/ml streptomycin), and viable large nucleated cells (95% in trypan blue exclusion test) were counted.


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