Words similar to trypan
Example sentences for: trypan
How can you use “trypan” in a sentence? Here are some example sentences to help you improve your vocabulary:
25%)-EDTA (1 mM) and trypan blue were purchased from GIBCO-BRL (Grand Island, NY); certified heat-inactivated fetal bovine serum (FBS) was obtained from Hyclone (Logan, UT); mouse anti-rat CD11b/c monoclonal antibody (OX-42) and rabbit anti-glutamate receptor (GluR4) polyclonal antibody were purchased from Pharmingen (San Diego, CA).
RPMI-1640 medium without phenol red, cell culture freezing medium containing DMSO, fetal bovine serum (FBS), penicillin, streptomycin, 7.5% bovine serum albumin (BSA), phosphate buffer saline (PBS, pH = 7.4), and trypan blue were purchased from Life Technologies (Grand Island, NY).
Placental tissue was digested and trophoblast cells isolated and cultured as described previously [ 15 36 ] . Briefly, the cells collected after digestion were pooled, washed twice in DMEM containing 25 mM HEPES and 4500 mg/L glucose (DMEM-HG), diluted to 10 ml in DMEM-HG supplemented with heat inactivated 5% fetal bovine serum (FBS) and antibiotics (50 μg/ml gentamycin, 100 U/ml penicillin, and 100 μg/ml streptomycin), and viable large nucleated cells (95% in trypan blue exclusion test) were counted.
Total numbers of viable cells at each mefloquine concentration were determined using trypan blue exclusion as previously described [ 14 ] . Viability (%) was calculated using the following formula: Viability (%) = # viable cells in treated culture/# viable cells in control culture * 100.
Samples for MTS and trypan blue exclusion assays were performed in triplicate and the data subjected to the Student's paired t -test analysis for determination of statistical significance between BRCA1+ and BRCA1wt samples.
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