Words similar to trypan
Example sentences for: trypan
How can you use “trypan” in a sentence? Here are some example sentences to help you improve your vocabulary:
On day 14 and every 3-4 days thereafter, microglia were detached using an orbital shaker (150 rpm, 0.5 hours, 36°C, 5% CO 2 ), centrifuged (400 × g, 25 min, 4°C), and microglia number and viability assessed by trypan blue exclusion.
Total numbers of viable cells at each mefloquine concentration were determined using trypan blue exclusion as previously described [ 14 ] . Viability (%) was calculated using the following formula: Viability (%) = # viable cells in treated culture/# viable cells in control culture * 100.
The number of viable GC in the monolayer cultures was determined by first harvesting the attached cells with a trypsin-containing solution (mg/ml): NaCl 8; KCl 0.4; dextrose 1; NaHCO 3 0.6; ethylenediaminetetraacetic acid sodium salt (Na-EDTA) 0.2; trypsin 0.5, and then counting directly the number of viable cells using trypan blue exclusion and hemacytometry.
In addition, the total number of viable cells in each treatment group was counted by a trypan blue exclusion method using a hemocytometer.
To determine the effect of the compounds on cell proliferation, after 24 h incubation, the cells were washed and further incubated in the absence of the compounds for 1 day, and then a trypan blue assay was done.
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