Words similar to trypan
Example sentences for: trypan
How can you use “trypan” in a sentence? Here are some example sentences to help you improve your vocabulary:
In an attempt to determine if increased survival of the antisense cells was do to increased proliferation of the antisense cells or decreased death rate, the number of trypan blue positive, non-viable were examined after 14 days without estrogen.
RPMI-1640 medium without phenol red, cell culture freezing medium containing DMSO, fetal bovine serum (FBS), penicillin, streptomycin, 7.5% bovine serum albumin (BSA), phosphate buffer saline (PBS, pH = 7.4), and trypan blue were purchased from Life Technologies (Grand Island, NY).
Tissues were then pinned flat on wax blocks and covered with trypan blue (0.
Cell viability was quantified by trypan blue exclusion analysis at the beginning of every experiment to ensure that cell cultures used in the experiments were healthy (alive cells >95%).
Placental tissue was digested and trophoblast cells isolated and cultured as described previously [ 15 36 ] . Briefly, the cells collected after digestion were pooled, washed twice in DMEM containing 25 mM HEPES and 4500 mg/L glucose (DMEM-HG), diluted to 10 ml in DMEM-HG supplemented with heat inactivated 5% fetal bovine serum (FBS) and antibiotics (50 μg/ml gentamycin, 100 U/ml penicillin, and 100 μg/ml streptomycin), and viable large nucleated cells (95% in trypan blue exclusion test) were counted.
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