Words similar to trypan

Example sentences for: trypan

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Total numbers of viable cells at each mefloquine concentration were determined using trypan blue exclusion as previously described [ 14 ] . Viability (%) was calculated using the following formula: Viability (%) = # viable cells in treated culture/# viable cells in control culture * 100.
25%)-EDTA (1 mM) and trypan blue were purchased from GIBCO-BRL (Grand Island, NY); certified heat-inactivated fetal bovine serum (FBS) was obtained from Hyclone (Logan, UT); mouse anti-rat CD11b/c monoclonal antibody (OX-42) and rabbit anti-glutamate receptor (GluR4) polyclonal antibody were purchased from Pharmingen (San Diego, CA).
Collected cells were pooled, washed twice in DMEM-HG, diluted to 10 ml in DMEM-HG supplemented with heat inactivated 5% fetal bovine serum (FBS; Gibco/BRL) and antibiotics (50 μg/ml gentamycin, 100 U/ml penicillin, and 100 μg/ml streptomycin), and viable large nucleated cells (95% in trypan blue exclusion test) were counted.
On day 14 and every 3-4 days thereafter, microglia were detached using an orbital shaker (150 rpm, 0.5 hours, 36°C, 5% CO 2 ), centrifuged (400 × g, 25 min, 4°C), and microglia number and viability assessed by trypan blue exclusion.
Cell viability, measured as trypan blue exclusion, varied from 98.