Example sentences for: trypan

How can you use “trypan” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • On day 14 and every 3-4 days thereafter, microglia were detached using an orbital shaker (150 rpm, 0.5 hours, 36°C, 5% CO 2 ), centrifuged (400 × g, 25 min, 4°C), and microglia number and viability assessed by trypan blue exclusion.

  • Total numbers of viable cells at each mefloquine concentration were determined using trypan blue exclusion as previously described [ 14 ] . Viability (%) was calculated using the following formula: Viability (%) = # viable cells in treated culture/# viable cells in control culture * 100.

  • The number of viable GC in the monolayer cultures was determined by first harvesting the attached cells with a trypsin-containing solution (mg/ml): NaCl 8; KCl 0.4; dextrose 1; NaHCO 3 0.6; ethylenediaminetetraacetic acid sodium salt (Na-EDTA) 0.2; trypsin 0.5, and then counting directly the number of viable cells using trypan blue exclusion and hemacytometry.

  • In addition, the total number of viable cells in each treatment group was counted by a trypan blue exclusion method using a hemocytometer.

  • To determine the effect of the compounds on cell proliferation, after 24 h incubation, the cells were washed and further incubated in the absence of the compounds for 1 day, and then a trypan blue assay was done.


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