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Example sentences for: tlk

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The Cy3 (HL60) and Cy5 (HL60/TLK286) PCR products were purified with Qiagen PCR purification kit, reduced to a final volume of 7.5 μl with a speed vac, mixed with 3.75 μl of 20x SSC, 0.75 μl of 10% SDS, 1.5 μl of 1 μg/μl salmon DNA and 1.5 μl of 50x Denhardt's solution, denatured at 95°C for 5 min, cooled on ice and incubated at 42°C for 15 min.
Fifteen μl each of the adapterized HL60 cDNA and HL60/TLK286 cDNA were mixed with 30 μl of 2x HB buffer in a final volume of 60 μl, denatured at 95°C for 5 min and annealed at 68°C for 20 hours.
The confidence levels of t tests reflect the magnitude of consistent differences between HL60 and HL60/TLK286 among replicates.
For example, the stress kinase JNK1 was activated during TLK286 induced apoptosis [ 17 ] and JNK is known to be activated during apoptosis induced by a variety of stress stimuli [ 18 ] . However, in HL60/TLK286 resistant cells, we observed an overexpression of two phosphatases PP2 (AA599092) and MKP5 (AA056608) involved in JNK dephosphorylation and inactivation (Table 2).
The total RNA samples of HL60 and HL60/TLK286 were reverse-transcribed with Superscript II reverse transcriptase.