Example sentences for: tlk
How can you use “tlk” in a sentence? Here are some example sentences to help you improve your vocabulary:
Briefly, twenty μg of total RNA from HL60 or HL60/TLK286 cells was reverse-transcribed into single stranded cDNA.
After phenol extraction and ethanol precipitation, the cDNA was resuspended in 25 μl of ddH 2 O. Both HL60 and HL60/TLK286 cDNA were digested with 3 μl (30 units) of the restriction enzyme Taq I in a final volume of 30 μl.
The Taq I fragments of HL60 cDNA were ligated with the CT adapter at 16°C overnight with 3 μl (9 units) of T4 ligase (Promega, Madison, WI) while the Taq I fragments of HL60/TLK286 cDNA were ligated with the TT adapter.
In addition, expression of GSTP1-1 was reduced in the HL60/TLK286 cell line, which was consistent with a previous study [ 7 ] . Quantitative RT-PCR confirmed the results of ADGE microarray for eleven of thirteen genes.
ADGE microarray was used to compare expression patterns for the TLK286 resistant cell line and its wild type counterpart by screening the expression pattern of 20,000 genes.