Example sentences for: tlk

How can you use “tlk” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • In addition, expression of GSTP1-1 was reduced in the HL60/TLK286 cell line, which was consistent with a previous study [ 7 ] . Quantitative RT-PCR confirmed the results of ADGE microarray for eleven of thirteen genes.

  • Briefly, ten μg of total RNA was reverse-transcribed into single stranded cDNA with oligo(dT) 12-18 . Then the double stranded cDNA for HL60 and HL60/TLK286 was generated with the cDNA Synthesis System (Life Technologies, Rockville, MD).

  • The denatured Cy3 (HL60) and Cy5 (HL60/TLK286) DNA were mixed and loaded onto a microarray chip.

  • The Taq I fragments of HL60 cDNA were ligated with the CT adapter at 16°C overnight with 3 μl (9 units) of T4 ligase (Promega, Madison, WI) while the Taq I fragments of HL60/TLK286 cDNA were ligated with the TT adapter.

  • PP2 is a serine/threonine phosphatase which dephosphorylates JNK during inflammatory cell signaling [ 19 ] , while MKP5 is a member of the dual specificity phosphatase family which selectively dephosphorylates stress activated MAP kinases including JNK [ 20 ] . The overexpression of these two phosphatases might contribute in part to the resistance of HL60/TLK286 by impairing the activity of JNK.


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