Words similar to tlk

Example sentences for: tlk

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Total RNA was isolated from both HL60 and HL60/TLK286 cell lines with a Qiagen RNeasy Midi Kit.
Although the interaction among GSTP1-1 and genes of proliferation and kinase pathways needs to be verified and the roles of other differential genes in the TLK286 resistant cell line need further investigation, the expression pattern generated with ADGE microarray provides a blueprint for designing further experiments.
Both HL60/TLK286 and HL60 cell lines were cultured in RPMI-1640 medium supplemented with 2 mM L-glutamine, 50 U/ml penicillin, 50 μg/ml streptomycin and 10% heat-inactivated fetal bovine serum.
Cellular response to chronic TLK286 exposure included a two-fold decrease in GSTP1-1 protein levels, confirming a GSTP1-1 mediated mechanism of activation [ 7 ] . Mouse embryo fibroblast cells from GSTP1-1 deficient animals elevated the expression of signal-regulated kinases ERK1/ERK2 and reduced the doubling time of cell proliferation [ 8 ] . That paper also found that a clone of HL60 cells resistant to TLK199, an inhibitor of GSTP1-1, has been shown to have elevated activities of c-Jun NH 2 -terminal kinase (JNK) and ERK1/ERK2.
Briefly, twenty μg of total RNA from HL60 or HL60/TLK286 cells was reverse-transcribed into single stranded cDNA.