Example sentences for: tlk

How can you use “tlk” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Although the interaction among GSTP1-1 and genes of proliferation and kinase pathways needs to be verified and the roles of other differential genes in the TLK286 resistant cell line need further investigation, the expression pattern generated with ADGE microarray provides a blueprint for designing further experiments.

  • After phenol extraction and ethanol precipitation, the cDNA was resuspended in 25 μl of ddH 2 O. Both HL60 and HL60/TLK286 cDNA were digested with 3 μl (30 units) of the restriction enzyme Taq I in a final volume of 30 μl.

  • Fifteen μl each of the adapterized HL60 cDNA and HL60/TLK286 cDNA were mixed with 30 μl of 2x HB buffer in a final volume of 60 μl, denatured at 95°C for 5 min and annealed at 68°C for 20 hours.

  • The HL60/TLK286 cell line is resistant to the prodrug TLK286 and derived from the wild-type HL60 by stepwise selection.

  • The Cy3 (HL60) and Cy5 (HL60/TLK286) PCR products were purified with Qiagen PCR purification kit, reduced to a final volume of 7.5 μl with a speed vac, mixed with 3.75 μl of 20x SSC, 0.75 μl of 10% SDS, 1.5 μl of 1 μg/μl salmon DNA and 1.5 μl of 50x Denhardt's solution, denatured at 95°C for 5 min, cooled on ice and incubated at 42°C for 15 min.


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