Example sentences for: tlk

How can you use “tlk” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Briefly, ten μg of total RNA was reverse-transcribed into single stranded cDNA with oligo(dT) 12-18 . Then the double stranded cDNA for HL60 and HL60/TLK286 was generated with the cDNA Synthesis System (Life Technologies, Rockville, MD).

  • The labeled HL60 cDNA and HL60/TLK286 cDNA were combined and purified.

  • In addition, GSTP1-1 acts as a negative regulator of JNK primarily through direct protein:protein interactions [ 9 10 ] . These data suggest that GSTP1-1 has a role in regulation of kinase activities and cell proliferation [ 8 11 12 13 ] . Because of this available background information, the HL60 wild type and TLK286 resistant cell lines were chosen as model systems for analysis by ADGE microarray.

  • The Cy3 (HL60) and Cy5 (HL60/TLK286) PCR products were purified with Qiagen PCR purification kit, reduced to a final volume of 7.5 μl with a speed vac, mixed with 3.75 μl of 20x SSC, 0.75 μl of 10% SDS, 1.5 μl of 1 μg/μl salmon DNA and 1.5 μl of 50x Denhardt's solution, denatured at 95°C for 5 min, cooled on ice and incubated at 42°C for 15 min.

  • Briefly, twenty μg of total RNA from HL60 or HL60/TLK286 cells was reverse-transcribed into single stranded cDNA.


How many words do you know? Try our free vocabulary size test!


Search

Search for example sentences

Loading Loading...
Quantcast