Example sentences for: tlk
How can you use “tlk” in a sentence? Here are some example sentences to help you improve your vocabulary:
Total RNA was isolated from both HL60 and HL60/TLK286 cell lines with a Qiagen RNeasy Midi Kit.
The denatured Cy3 (HL60) and Cy5 (HL60/TLK286) DNA were mixed and loaded onto a microarray chip.
Fifteen μl each of the adapterized HL60 cDNA and HL60/TLK286 cDNA were mixed with 30 μl of 2x HB buffer in a final volume of 60 μl, denatured at 95°C for 5 min and annealed at 68°C for 20 hours.
After phenol extraction and ethanol precipitation, the cDNA was resuspended in 25 μl of ddH 2 O. Both HL60 and HL60/TLK286 cDNA were digested with 3 μl (30 units) of the restriction enzyme Taq I in a final volume of 30 μl.
The labeled HL60 cDNA and HL60/TLK286 cDNA were combined and purified.
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