Example sentences for: thp-

How can you use “thp-” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • THP-1 (generously provided by Dr P Guyre, Dartmouth Medical School), Jurkat (ATCC), and 293 cell lines were cultured in RPMI complete medium (CM), consisting of RPMI 1640 with 10% heat-inactivated fetal bovine serum, 0.1 ml of sodium pyruvate, 2.0 mmol/l of L-glutamine, 25 mmol/l of 1 M HEPES (N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid), 0.1 mmol/l of nonessential amino acids, 0.25 μg/ml of amphotericin, 50 μg/ml gentamicin (all from BioWhittaker, Walkersville, MD, USA) and 5 × 10 -5M 2-mercaptoethanol (Sigma, St Louis, MO, USA).

  • The multiplicity of infection (MOI) was ~500 bacteria per THP-1 cell.

  • Another reported mechanism of IFNg resistance in tumor cells is associated with down-regulation of IFNg-receptors [ 38 ] . The relevance of this mechanism to MHCII inducibility was recently supported by the evidence that PMA is able to enhance IFNg-dependent MHCII expression in THP-1 human leukemia cells through the up-regulation of IFNg receptors [ 19 ] . In our experiments, however, the incubation of LS1034 cells with PMA and ethanol did not lead to any changes in IFNgR expression as determined by flow cytometry (Figure 4).

  • In the studies reported here, we used a similar enrichment scheme to identify two additional recombinant M. smegmatis clones, sur2 and sur3, that demonstrated enhanced survival during infections of THP-1 human monocyte-derived macrophages.

  • It has been previously shown that potentiating effect of phorbol esters on IFNg-dependent MHCII induction in THP-1 monocytic cell line was associated with the increase in synthesis of IFNg receptors [ 19 ] . For that reason, we questioned whether PMA could produce similar changes in LS1034 colon carcinoma cells.


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