Words similar to thiamine
Example sentences for: thiamine
How can you use “thiamine” in a sentence? Here are some example sentences to help you improve your vocabulary:
This finding suggests that cellular and mitochondrial uptake of thiamine may be mediated by the same transporter since TRMA is defined by mutation within the thiamine transporter located on the plasma membrane [ 7 8 9 10 11 12 ] . Using antiserum specific for the human thiamine transporter that is mutated in TRMA individuals, western analysis consistently resulted in a faint but detectable band within the isolated mitochondrial suspension (fig.
Interestingly, mitochondria from TRMA lymphoblasts did not possess a "high affinity" thiamine transport capacity as did mitochondria form normal lymphoblasts.
Thiamine entry into mammalian cells occurs by a saturable, high affinity transporter that is deficient in humans with thiamine-responsive megaloblastic anemia (TRMA) [ 7 8 9 10 11 12 ] . Thiamine uptake by mitochondria has been demonstrated [ 13 ] , yet thiamine diphosphokinase is cytosolic and mitochondria cannot convert thiamine to ThDP [ 14 15 16 ] . Barile and coworkers demonstrated saturable uptake of ThDP by rat liver mitochondria characterized by a K m of around 20 μM.
Interestingly, ThDP-utilizing enzymes in mitochondria are much less affected (as revealed by loss of activity) upon progressive depletion of thiamine available to TRMA cells than are ThDP-utilizing enzymes of the cytosol [ 6 ] . For these reasons, we have examined the uptake of thiamine and especially ThDP by mitochondria from several human cell types, including cells from TRMA patients.
CKS conceived of the study, participated in its design and coordination, performed a few of the thiamine uptake by cells experiments, and participated in writing the manuscript.
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