Words similar to thif
Example sentences for: thif
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Phylogenic trees were inferred using PAUP 4.0 [ 44 ] . Three data sets were analyzed: those portions of the NA alignment coding for the ThiF domains of the MoeZ conceptual proteins, the alignment of the complete MoeZ sequences, and the alignment of the full length 16S rDNA (SSU) sequences.
The incongruence between the SSU and the ThiF domain trees suggests that Ps-moeZ may have been acquired by P. stutzeri strain KC by lateral gene transfer (LGT) from an ancestor more closely related to mycobacteria and cyanobacteria than to proteobacteria.
This dual CXXC motif, found in MoeB, ThiF, and HesA proteins, forms a metal center with a zinc atom [ 7 9 ] . The presence of all four of these cysteines has been shown to be required for activity in MoeB).
All 12 sequences in this region have high similarity to the derived consensus sequence GXGXXG(7X)A(SX)GXGXXG(4X)D(9X)R, which defines a nucleotide binding motif [ 16 25 ] . In addition, all sequences have five residues, or conservative substitutions in the case of Pa-MoeB, shown to participate in the adenylation reaction catalyzed by Ec-MoeB (Figure 3) [ 7 ] . The fact that such high similarities are retained in the ThiF coding region across three bacterial divisions suggests that this domain functions more or less autonomously.
MoeBR has the ThiF and 2X CXXC domains configured like MoeB but includes an N-terminal rhodanese-like domain, the polyproline motif, and has a Y substitution at position 155 (Ec-MoeB numbering).