Words similar to terminators

Example sentences for: terminators

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The genes comprising the supraoperon of B. subtilis are aroG → aroB → aroH → trpAaBDCEbEa → hisH b → tyrA p → aroF . A hierarchy of internal promoters and terminators exists for differential control of the B. subtilis supraoperon.
Genomic DNA and total RNA were extracted from fibroblast cultures established from skin biopsies as described [ 14 ] . Long-range RT-PCR amplification of FBN1 cDNA fragments and restriction enzyme digestions were performed as previously reported [ 8 ] . Fragments migrating abnormally in agarose gel electrophoresis were excised, purified with the QIAquick Gel Extraction Kit (QIAGEN) and directly sequenced with fluorescent terminators on an ABI Prism 377 DNA sequencer (Perkin-Elmer).
All three BACs were fragmented by ultrasound, shotgun cloned into the vector pBluescript-KS, and extensively sequenced using ABI model 373 and 377 sequencers, using Big Dye terminators; 959 reads were obtained from 390I17, 1503 reads from 110P15, and 2731 reads from 152C16.
The annealing temperatures for each primer pair are listed in Table 1. Sequencing was with dye-labeled terminators (ABI 377 Sequencer).
The 16S rDNA library was constructed as previously described [ 11 ] . The clones in the libraries were sequenced once from the 5' end using Bact27F (ABI PRISM BigDye Terminators on an ABI377XL sequencer (PE Applied Biosystems, Inc.; Foster City, CA) at the San Diego State University Microchemical Core Facility).