Words similar to synthesizing
Example sentences for: synthesizing
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Plasmid construction for synthesizing riboprobes
cDNA microarrays are created by spotting amplified cDNA fragments in a high density pattern onto a solid substrate such as a glass slide [ 1 2 ] . Oligonucleotide arrays are either spotted or constructed by chemically synthesizing approximately 25-mer oligonucleotide probes directly onto a glass or silicon surface using photolithographic technology [ 3 ] .
The cDNA array has a long history of development [ 3 ] stemming from immunodiagnostic work in the 1980s; however, it has been most widely developed in recent years by Stanford University (California) researchers depositing cDNA tags onto glass slides, or chips, with precise robotic printers [ 4 ] . Labeled cDNA fragments are then hybridized to the tags on the chip, scanned, and differences in mRNA between samples identified and visualized using a variation of the red/green matrix originally introduced by Eisen and colleagues [ 5 ] . The light-generated oligonucleotide array, developed by Affymetrix, Inc. (Santa Clara, CA), involves synthesizing short 25-mer oligonucleotide probes directly onto a glass slide using photolithographic masks [ 6 7 ] . Sample processing includes the production of labeled cRNA, hybridization to a microarray, and quantification of the obtained signal after laser scanning.
3A) may be due to the use of small format gels or possibly to the usage of the second N-terminal methionine by the cell for synthesizing human myocilin, making human and monkey molecular weights comparable.
While Lerisetron contains no carbonyl group, the second nitrogen contained in the benzimidazole heterocycle could act as bioisostere of this functional group [ 22 ] . Orjales demonstrated the importance of the N-benzyl group by synthesizing several N1 substituted analogs of Lerisetron.
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