Example sentences for: supernatants

How can you use “supernatants” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Indeed, at 4 hours post-infection, Gro-1 was induced, as measured by enzyme-linked immunosorbent assay (ELISA), and was detected at similar levels in the supernatants following infections with each of the three genotypes tested, as predicted by the array experiments (Figure 9a).

  • The supernatants were removed, and placed on ice.

  • After 10 min on ice, lysates were centrifuged (4000 × g, 5 min) and supernatants containing 10 μg of protein fractionated on 10% SDS polyacrylamide gels.

  • After 30 min, cell lysates were centrifuged (16,500 × g for 20 min) and cytoplasmic DNA extracted from the supernatants by overnight precipitation in 0.1× volume 3 M sodium acetate (pH 8.0) and 2× volume 100% ethanol.

  • To prepare cell lysates, freshly isolated trophoblast cells and early (16-24 h) and late trophoblast cultures (72-96 h) were washed twice with ice-cold PBS, lysed by adding ice-cold lysis buffer (20 mM Tris pH 7.5, 200 mM NaCl) containing 1 mM sodium orthovanadate, 0.25% Nonidet P-40, 10 mM sodium fluoride, and 1 mM phenylmethylsulfonyl fluoride, sonicated, centrifuged, and supernatants stored at -80°C until use [ 15 ] .


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