Words similar to supernatants
Example sentences for: supernatants
How can you use “supernatants” in a sentence? Here are some example sentences to help you improve your vocabulary:
Indeed, at 4 hours post-infection, Gro-1 was induced, as measured by enzyme-linked immunosorbent assay (ELISA), and was detected at similar levels in the supernatants following infections with each of the three genotypes tested, as predicted by the array experiments (Figure 9a).
The supernatants were removed, and placed on ice.
After 10 min on ice, lysates were centrifuged (4000 × g, 5 min) and supernatants containing 10 μg of protein fractionated on 10% SDS polyacrylamide gels.
After 30 min, cell lysates were centrifuged (16,500 × g for 20 min) and cytoplasmic DNA extracted from the supernatants by overnight precipitation in 0.1× volume 3 M sodium acetate (pH 8.0) and 2× volume 100% ethanol.
To prepare cell lysates, freshly isolated trophoblast cells and early (16-24 h) and late trophoblast cultures (72-96 h) were washed twice with ice-cold PBS, lysed by adding ice-cold lysis buffer (20 mM Tris pH 7.5, 200 mM NaCl) containing 1 mM sodium orthovanadate, 0.25% Nonidet P-40, 10 mM sodium fluoride, and 1 mM phenylmethylsulfonyl fluoride, sonicated, centrifuged, and supernatants stored at -80°C until use [ 15 ] .