Example sentences for: supernatants

How can you use “supernatants” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • After 10 min on ice, lysates were centrifuged (4000 × g, 5 min) and supernatants containing 10 μg of protein fractionated on 10% SDS polyacrylamide gels.

  • To prepare cell lysates, freshly isolated trophoblast cells and early (16-24 h) and late trophoblast cultures (72-96 h) were washed twice with ice-cold PBS, lysed by adding ice-cold lysis buffer (20 mM Tris pH 7.5, 200 mM NaCl) containing 1 mM sodium orthovanadate, 0.25% Nonidet P-40, 10 mM sodium fluoride, and 1 mM phenylmethylsulfonyl fluoride, sonicated, centrifuged, and supernatants stored at -80°C until use [ 15 ] .

  • Supernatants of all cell lines attracted significantly more monocytes than medium alone ( P < 0.01).

  • Supernatants were taken at 5, 10, and 15 days and stored at -20°C for later analysis.

  • Following the incubation period, samples were centrifuged at 9 000 × g for 30 seconds and the supernatants collected.


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