Example sentences for: supernatants

How can you use “supernatants” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • 10 6cells/ml were plated in a 24 well culture dish for 24 hr in tumor cell or control cell conditioned media then supernatants were assayed for IL10 by ELISA (R&D).

  • For immunoabsorption of viral proteins, the culture supernatants were incubated for 60 min with protein A beads conjugated with polyclonal antisera to either Tat or gp120 with constant shaking at room temperature.

  • Enzyme activity in aliquots (50 μl) of the supernatants was determined using the LD-L kit from Sigma.

  • BG-1 human ovarian adenocarcinoma cells were infected with a retroviral construct composed of an antisense 900 base-pair cDNA sequence of the amino-terminal region of BRCA1 . Three experiments (two of which were from independently made supernatants) showed that infection of pLXSN (vector alone) and BRCA1 antisense retroviral constructs into BG-1 cells yielded G418-resistant colonies at similar rates (titers ranged from 0.78 to 4.2 × 10 4colony-forming units/ml).

  • To the cytosolic fractions (supernatants), glycerol was added to a final concentration of 40%.


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