Example sentences for: supernatant

How can you use “supernatant” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Concentration of supernatant to viral titers exceeding 10 7cfu/ml appeared to have the greatest quantitative impact on improving transduction efficiency.

  • A total of 2 × 10 5NIH 3T3 cells were transduced with serial dilutions of supernatant.

  • The scraped cells were centrifuged at 2000 × g for 5 min at 4°C, the supernatant was discarded, and the pellet was stored at -80°C until assayed.

  • In the presence of 50, 100, and 200 mM additional KCl, the ratio of Boi1-PH that pelleted with respect to that which stayed in the supernatant was greater when using vesicles that contained PIP 2 (Fig.

  • It has previously been shown that IL-8 mRNA induction was seen less then 1 h after Tat (72aa) stimulation, and levels remained elevated for up to 24 h, leading to IL-8 protein production [ 102 ] . Along these lines, we have previously shown that the IL-8 gene is expressed in a cell cycle-dependent manner in cells that express the Tat protein, and the induction is during the S phase of the cell cycle and regulated by stable NF-kB binding to the IL-8 promoter [ 103 ] . When looking for IL-8 at the G1/S border, we found that all Tat containing cells, including PBMCs that were treated with exogenous Tat showed an up-regulation of IL-8 in the supernatant (Figure 4), further implying that results obtained from the H9/Tat system may infact be of general physiological relevance in vivo.


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