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Mouse monoclonal Ab SMI52 to microtubule-associated protein 2 (Sternberger Monoclonals, Baltimore, MD) was used at a concentration of 2 μg/ml, followed by the rhodamine goat anti-mouse secondary.
SH-SY5Y cells are a subclone of the SK-N-SH human neuroblastoma cell line derived from neoplastic neural crest cells and under certain growth conditions, generate neuritic processes [ 12 13 ] . Sternberger and Sternberger [ 14 ] describe SMI-31 mAB as specific for phosphorylated epitopes on the heavy neurofilaments peptide (NF-H) and to a lesser extent medium neurofilament peptide (NF-M).
Dendritic growth was assessed in cultures immunostained with monoclonal antibodies (mAb) previously shown to react selectively with the somatodendritic compartment of cultured sympathetic neurons [ 16 ] . These mAb's include SMI-52, which is specific for the cytoskeletal protein MAP2, and SMI-32, which reacts with the non-phosphorylated forms of the M and H neurofilament subunits (Sternberger Immunocytochemicals, Baltimore, MD).