Example sentences for: sspe-t

How can you use “sspe-t” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The probe array was incubated for 16 h at 40°C at constant rotation (60 rpm), then exposed to 10 washes in 6X SSPE-T at 25°C followed by 4 washes in 0.5X SSPE-T at 50°C.

  • Prior to hybridization, fragmented cRNA in a 6X SSPE-T hybridization buffer (6X: 1M NaCl, 10mM Tris pH 7.6, 0.005% Triton), was heated to 95°C for 5 min, cooled to 40°C and loaded onto an Affymetrix probe array cartridge.

  • Biotinylated cRNA was stained with 10 g/mL streptavidin-phycoerythrin conjugate (Molecular Probes) in 6X SSPE-T for 30 min at 25°C followed by 10 washes in 6xSSPE-T at 25°C.

  • The biotinylated cRNA was stained with a streptavidin-phycoerythrin conjugate, 10 g/ml (Molecular Probes) in 6×SSPE-T for 30 min at 25°C followed by 10 washes in 6×SSPE-T at 25°C.

  • The probe array was washed 10× in 6×SSPE-T at 25°C followed by 4 washes in 0.5×SSPE-T at 50°C.


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