Words similar to sspe
Example sentences for: sspe
How can you use “sspe” in a sentence? Here are some example sentences to help you improve your vocabulary:
5% SDS at 55°C for 30 min, and twice with 0.1X SSPE at room temperature for 10 min.
0. STE buffer gave background problems possibly because the pH of this buffer tended to decrease as temperature increased whereas SSC and SSPE better retained pH.
SSC, SSPE and STE buffers at pH 6.0, 6.5, 7.0, 7.5 and 8.0 were tested as variables of the protocol listed in Table 1. After pealing away rubber cement, slides were immersed in 2X buffer/0.
The membrane was washed twice with 2X SSPE at room temperature for 10 min, three times with 0.1X SSPE/0.
The membrane was prehybridized for 2 hours at 42°C in 10 ml of hybridization buffer (final concentrations: 50% formamide, 5X Denhardt's solution, 0.1% SDS, 5X SSPE, 150 μg/ml denatured herring sperm DNA), and hybridized with the denatured probe overnight in 5 ml of hybridization buffer at 42°C.
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