Words similar to sspe
Example sentences for: sspe
How can you use “sspe” in a sentence? Here are some example sentences to help you improve your vocabulary:
SSC, SSPE and STE buffers at pH 6.0, 6.5, 7.0, 7.5 and 8.0 were tested as variables of the protocol listed in Table 1. After pealing away rubber cement, slides were immersed in 2X buffer/0.
Nylon membranes were stripped by washing twice for 10 min in boiling 0.01X SSPE (0.
200 μL of annealing buffer (6x SSPE, 0.01% Triton X-100) containing 0.5 μM primer (Table 2) was then pipeted onto the gel and covered with a hybrid well chamber.
The hybridization was carried out essentially as described [ 62 ] . After hybridization, the membranes were washed twice at 80°C in 0.2× SSPE and 0.5% SDS for 30 minutes, and subsequently exposed to Kodak X-AR autoradiographic film.
The membrane was prehybridized for 2 hours at 42°C in 10 ml of hybridization buffer (final concentrations: 50% formamide, 5X Denhardt's solution, 0.1% SDS, 5X SSPE, 150 μg/ml denatured herring sperm DNA), and hybridized with the denatured probe overnight in 5 ml of hybridization buffer at 42°C.
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