Words similar to sspe

Example sentences for: sspe

How can you use “sspe” in a sentence? Here are some example sentences to help you improve your vocabulary:

200 μL of annealing buffer (6x SSPE, 0.01% Triton X-100) containing 0.5 μM primer (Table 2) was then pipeted onto the gel and covered with a hybrid well chamber.
The hybridization was carried out essentially as described [ 62 ] . After hybridization, the membranes were washed twice at 80°C in 0.2× SSPE and 0.5% SDS for 30 minutes, and subsequently exposed to Kodak X-AR autoradiographic film.
0. STE buffer gave background problems possibly because the pH of this buffer tended to decrease as temperature increased whereas SSC and SSPE better retained pH.
The membrane was prehybridized for 2 hours at 42°C in 10 ml of hybridization buffer (final concentrations: 50% formamide, 5X Denhardt's solution, 0.1% SDS, 5X SSPE, 150 μg/ml denatured herring sperm DNA), and hybridized with the denatured probe overnight in 5 ml of hybridization buffer at 42°C.
5 mM NaCl) or in hybridization buffer (50% formamide/6 × SSPE), then the melting profile was performed in 1°C increments with constant monitoring at 260 nm.