Words similar to sspe
Example sentences for: sspe
How can you use “sspe” in a sentence? Here are some example sentences to help you improve your vocabulary:
5 mM NaCl) or in hybridization buffer (50% formamide/6 × SSPE), then the melting profile was performed in 1°C increments with constant monitoring at 260 nm.
0. STE buffer gave background problems possibly because the pH of this buffer tended to decrease as temperature increased whereas SSC and SSPE better retained pH.
The hybridization was carried out essentially as described [ 62 ] . After hybridization, the membranes were washed twice at 80°C in 0.2× SSPE and 0.5% SDS for 30 minutes, and subsequently exposed to Kodak X-AR autoradiographic film.
SSC, SSPE and STE buffers at pH 6.0, 6.5, 7.0, 7.5 and 8.0 were tested as variables of the protocol listed in Table 1. After pealing away rubber cement, slides were immersed in 2X buffer/0.
There was little difference between SSC and SSPE buffers.