Example sentences for: sspe

How can you use “sspe” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • 200 μL of annealing buffer (6x SSPE, 0.01% Triton X-100) containing 0.5 μM primer (Table 2) was then pipeted onto the gel and covered with a hybrid well chamber.

  • SSC, SSPE and STE buffers at pH 6.0, 6.5, 7.0, 7.5 and 8.0 were tested as variables of the protocol listed in Table 1. After pealing away rubber cement, slides were immersed in 2X buffer/0.

  • There was little difference between SSC and SSPE buffers.

  • The membrane was prehybridized for 2 hours at 42°C in 10 ml of hybridization buffer (final concentrations: 50% formamide, 5X Denhardt's solution, 0.1% SDS, 5X SSPE, 150 μg/ml denatured herring sperm DNA), and hybridized with the denatured probe overnight in 5 ml of hybridization buffer at 42°C.

  • The membrane was washed twice with 2X SSPE at room temperature for 10 min, three times with 0.1X SSPE/0.


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