Example sentences for: sspe

How can you use “sspe” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The membrane was washed twice with 2X SSPE at room temperature for 10 min, three times with 0.1X SSPE/0.

  • The hybridization was carried out essentially as described [ 62 ] . After hybridization, the membranes were washed twice at 80°C in 0.2× SSPE and 0.5% SDS for 30 minutes, and subsequently exposed to Kodak X-AR autoradiographic film.

  • There was little difference between SSC and SSPE buffers.

  • Nylon membranes were stripped by washing twice for 10 min in boiling 0.01X SSPE (0.

  • The membrane was prehybridized for 2 hours at 42°C in 10 ml of hybridization buffer (final concentrations: 50% formamide, 5X Denhardt's solution, 0.1% SDS, 5X SSPE, 150 μg/ml denatured herring sperm DNA), and hybridized with the denatured probe overnight in 5 ml of hybridization buffer at 42°C.

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