Example sentences for: sspe

How can you use “sspe” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • 200 μL of annealing buffer (6x SSPE, 0.01% Triton X-100) containing 0.5 μM primer (Table 2) was then pipeted onto the gel and covered with a hybrid well chamber.

  • SSC, SSPE and STE buffers at pH 6.0, 6.5, 7.0, 7.5 and 8.0 were tested as variables of the protocol listed in Table 1. After pealing away rubber cement, slides were immersed in 2X buffer/0.

  • Nylon membranes were stripped by washing twice for 10 min in boiling 0.01X SSPE (0.

  • The hybridization was carried out essentially as described [ 62 ] . After hybridization, the membranes were washed twice at 80°C in 0.2× SSPE and 0.5% SDS for 30 minutes, and subsequently exposed to Kodak X-AR autoradiographic film.

  • 5% SDS at 55°C for 30 min, and twice with 0.1X SSPE at room temperature for 10 min.


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