Words similar to spliced
Example sentences for: spliced
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Similarly to human SLC4A9, the rabbit gene is alternatively spliced.
1%) were guanosine at this position in the splice sequence making it likely that exon 68 will be aberrantly spliced [ 23 ] . When these two fragments were run on the DHPLC system at the lower second pass temperatures indicated in Additional file A: Primer sequences and DHPLC temperatures Excel spreadsheet, the alterations were detected (Figure 2cand 2b).
PCR products were restriction digested with BamH1 (exon 1) or Not1 (exon 2), purified from an agarose gel by Qiaquick extraction (Qiagen), and simultaneously subcloned into a BamH1/Not1 digested pcDNA3 (Invitrogen) vector to create a directional clone of rabbit MYOC exons 1 and 2 spliced together (pcDNA3.RbMYOCex1-2).
To locate exon-intron positions in previously uncharacterised genes (or predicted genes) that have not yet been associated with cDNAs, we performed spliced alignment, maintaining maximum similarity between homologues, as described in Methods.
found that a SV40 small-t intron only 66 nucleotides in length was spliced efficiently in tobacco cells [ 66].