Words similar to spheroplasts

Example sentences for: spheroplasts

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The spheroplasts were pelleted by centrifugation at 16,000 rpm for 10 min at 4°C.
Following induction of the λ repressor fragment, whole cell lysates, spheroplasts, and periplasmic fractions were prepared from each strain.
The strain is a derivative of VL6-48 with the ura3-52 gene replaced by the KanMX cassette [ 15 ] . Spheroplasts were generated as described previously [ 29 ] . Agarose plugs (60 ul) containing approximately 2 ug of high molecular weight DNA were prepared from normal human fibroblasts MRC-5 (ATCC) or from liver cells of the Tg.AC mouse [ 11 ] . Linearized TAR cloning vector (1 ug) was added to the plugs, treated with agarase, and mixed with yeast spheroplasts.
Yield of the transformants varied from 100 to 300 colonies/ug vector, 2 ug genomic DNA, and 2 × 10 8spheroplasts (Table 3).
The vector was linearized between the B1 targeting hook and URA3 and co-transformed with genomic DNA into yeast spheroplasts.