Words similar to spheroplasts

Example sentences for: spheroplasts

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The resulting spheroplasts were attached to poly-L-lysine coated coverslips and treated with cold methanol, then acetone.
The targeting hooks were placed in the promoter region of URA3 as shown in Figure 2. Genomic DNA and the linearized TAR cloning vector were combined with yeast spheroplasts and the His +Ura -transformants were selected on medium containing 5-FOA to identify clones with genomic DNA inserts.
The spheroplasts were pelleted by centrifugation at 16,000 rpm for 10 min at 4°C.
Following induction of the λ repressor fragment, whole cell lysates, spheroplasts, and periplasmic fractions were prepared from each strain.
Yield of the transformants varied from 100 to 300 colonies/ug vector, 2 ug genomic DNA, and 2 × 10 8spheroplasts (Table 3).