Example sentences for: spheroplasts

How can you use “spheroplasts” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The vector was linearized between the B1 targeting hook and URA3 and co-transformed with genomic DNA into yeast spheroplasts.

  • The spheroplasts were pelleted by centrifugation at 16,000 rpm for 10 min at 4°C.

  • The resulting spheroplasts were attached to poly-L-lysine coated coverslips and treated with cold methanol, then acetone.

  • The targeting hooks were placed in the promoter region of URA3 as shown in Figure 2. Genomic DNA and the linearized TAR cloning vector were combined with yeast spheroplasts and the His +Ura -transformants were selected on medium containing 5-FOA to identify clones with genomic DNA inserts.

  • Following induction of the λ repressor fragment, whole cell lysates, spheroplasts, and periplasmic fractions were prepared from each strain.


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