Words similar to spheroplasts

Example sentences for: spheroplasts

How can you use “spheroplasts” in a sentence? Here are some example sentences to help you improve your vocabulary:

The strain is a derivative of VL6-48 with the ura3-52 gene replaced by the KanMX cassette [ 15 ] . Spheroplasts were generated as described previously [ 29 ] . Agarose plugs (60 ul) containing approximately 2 ug of high molecular weight DNA were prepared from normal human fibroblasts MRC-5 (ATCC) or from liver cells of the Tg.AC mouse [ 11 ] . Linearized TAR cloning vector (1 ug) was added to the plugs, treated with agarase, and mixed with yeast spheroplasts.
Following induction of the λ repressor fragment, whole cell lysates, spheroplasts, and periplasmic fractions were prepared from each strain.
The resulting spheroplasts were attached to poly-L-lysine coated coverslips and treated with cold methanol, then acetone.
The targeting hooks were placed in the promoter region of URA3 as shown in Figure 2. Genomic DNA and the linearized TAR cloning vector were combined with yeast spheroplasts and the His +Ura -transformants were selected on medium containing 5-FOA to identify clones with genomic DNA inserts.
The vector was linearized between the B1 targeting hook and URA3 and co-transformed with genomic DNA into yeast spheroplasts.