Example sentences for: spheroplast

How can you use “spheroplast” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • To ensure that all periplasmic contents were released during the spheroplast preparation, the blots were stripped of FLAG antibody and reprobed with antibody directed against MalE, a periplasmic binding protein.

  • 5 by addition of 1 mM IPTG either for 30 minutes (spheroplast procedures) or for 3 hours (immunoprecipitation experiments).

  • The cell pellet was resuspended in 0.5 ml spheroplast buffer [1 M sucrose, 25 mM TrisHCl (pH 8.0), 5 mM EDTA, and lysozyme (50 μg ml -1)].

  • The pellet, which consisted of the membranes and cytoplasmic contents, was resuspended in 0.5 ml of spheroplast buffer.


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