Words similar to spei

Example sentences for: spei

How can you use “spei” in a sentence? Here are some example sentences to help you improve your vocabulary:

The wild type plasmid pTZtd1304 [ 17 ] contains a fully functional 265 nt derivative of the 1016 nt wild type intron [ 15 ] . This derivative also contains the substitutions U34A, which introduces a SpeI site, and U976G, which introduces an EcoRI site.
The resulting double-stranded DNA was diluted 1:50 and amplified using primers SpeI.
The PCR product was digested with SpeI (New England Biolabs, Beverly, MA; 20 units) and EcoRI (50 units) in buffer (50 mM NaCl, 100 mM Tris-HCl, pH 7.5, 10 mM MgCl 2 , 0.025% Triton X-100, 100 μg/ml BSA) at 37°C for 60 minutes, purified, and cloned into SpeI/EcoRI digested pTZtd1304.
To construct [ pMET3-CDC15, URA3 ] (pWS100) and [ pMET3-cdc15-2, URA3 ] (pWS109), the corresponding genes (from start codon to 300 bps downstream of the stop codon) were amplified by PCR from genomic DNA of wild type (RJD381) or cdc15-2 (RJD619) cells, and cloned into the SpeI and SacII sites of RDB620 (a derivative of pRS316 containing the MET3 promoter inserted between the HindIII and EcoRI sites).
The inserts were released by EcoRI and SpeI digestion and sized using agarose gel electrophoresis.