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Example sentences for: spe

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pBS-KS +-NFATp was generated by digesting pREP-NFATp with BssH II and Dra I, filling in the ends with Klenow, and ligating the resulting fragment into the Sma I site of pBS-KS +. pVL1392-HAX-NFATp was generated by subcloning a fragment excised from pBS-KS +-NFATp with Spe I and EcoR I into pVL1392-HAX (gift of S. Ruppert and R. Tjian) digested with Spe I and EcoR I. pBS-KS +-NFATp(NdeI), which has an Nde I site, containing the NFATp translational start site ATG, was generated in two steps.
The probes were: for SUC2, the 1.3 kb Bam HI- Hind III fragment of pRB59 [ 31]; for GAL1-10, the 2 kb Eco RI- Eco RI fragment of BNN45 [ 32] and for SPT15, the 0.8 kb Spe I- Hind III fragment of pIP45 (I.
The PCR product was digested with the restriction enzymes Xba I and Spe I and cloned into Xba I-digested pGEM-IIZf (Promega).
Bond Elut C 8 SPE cartridges (1 ml/100 mg) were purchased from Varian/Sample Preparation Products (Harbor City, CA, USA).