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Example sentences for: speb

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Analysis of culture supernatants for the presence of SpeB (Fig.
Organisms selected in this model are consistently negative for SpeB secretion.
Previous studies from our laboratory have consistently demonstrated loss of the SpeB phenotype in S. pyogenes isolates injected in a skin air sac and recovered from the organs of lethally infected mice [ 10 16 ] . This selection was not associated with loss of β-hemolysis but was associated with over-expression of M and M-related proteins, which in turn are predictive of the invasive potential of the organism in a skin infection model [ 16 ] . Based on the phenotypic characteristics of the kidney-recovered variant (β-hemolytic positive, M and M-related protein positive and SpeB negative) we predicted that this variant would be as virulent or more virulent than the wild type organism in the mouse skin infection model.
Selection of SpeB negative variants have also been noted following sequential human blood passage of isolates or in a mouse skin infection model [ 39 41 ] . This selective pressure can also be associated with enhanced capsular expression in SpeB negative variants [ 42 ] .
The sagA/pel mutant of isolate CS101 fails to express β-hemolysin, SpeB, SK or surface M and M-related proteins [ 4 ] . Based on prior studies from our laboratory [ 9 10 ] , we predicted that this isolate would be avirulent in the mouse skin air sac model.