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Example sentences for: smad-

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Phosphorylation of Smad-1 is evident within 30 min after exposure to BMP-5 (Figure 4A) with significantly increased pSmad-1 levels apparent at 60 min.
In contrast, treatment with BMP-5 did not alter levels of total Smad-1 (Figure 4B).
In most cell systems that have been examined, the primary signal transduction pathway for BMPs involves phosphorylation of Smad-1 [ 22 ] , and recent evidence suggests that this is also true for BMP-7 induced dendritic growth in sympathetic neurons [ 23 ] . To determine if BMP-5 initiates similar signaling events in sympathetic neurons, cultured sympathetic neurons grown in the absence of glial cells were treated with BMP-5 for 30 or 60 min then analyzed by Western blotting using Abs specific for total Smad-1 or for phosphorylated Smad-1.
Ab specific for the phosphorylated form of Smad-1 (Ser 463/465) as well as Ab that recognizes both phosphorylated and nonphosphorylated Smad-1 (e.g.
It has been shown that phosphorylation of Smad-1 precedes dendritic extension in cultured sympathetic neurons exposed to BMP-7; moreover, expression of a dominant negative construct of Smad-1 in cultured sympathetic neurons significantly inhibits BMP-7-induced dendritic growth in these neurons [ 23 ] . These data suggest that activation of Smad-1 is a necessary component of the signal transduction pathway by which BMP-7 induces dendritic growth.