Words similar to serum-free

Example sentences for: serum-free

How can you use “serum-free” in a sentence? Here are some example sentences to help you improve your vocabulary:

Briefly, rat neonatal microglia or cerebellar granule cells (60,000 cells / well) were plated in LPS-free 96-well cell culture clusters 24 hours prior to the experiments in either 0.1 ml DMEM supplemented with 10% FBS + 120 U/ml P + 12 μg/ml S (microglia), or 0.1 ml serum-free Neurobasal A medium with B27 supplement (cerebellar granule neurons).
To determine whether TGFβ1 could eliminate differences in α-SMA levels between PP and non-PP fibroblasts, cultures were incubated at confluence for three days with serum-free or serum-containing media supplemented with 0 ng/ml or 10 ng/ml active TGFβ1, and α-SMA and apoptosis were evaluated (Fig.
Subconfluent cells (5 × 10 3cells/cm 2) were transfected with 2 μg of the relevant plasmid using Fugene-6 (Boehringer Mannheim) in DMEM containing 0.2% BSA, 100 U/ml penicillin, 100 μg/ml streptomycin, 0.292 mg/ml glutamine (serum-free medium) following the manufacturer's protocol.
However, in the present model of SW1353 cells under serum-free conditions, we did not observe a mitogenic effect of IL-1 (data not shown).
The medium was changed to serum-free medium (1% BSA/DMEM) supplemented with 50 μg/ml of ascorbic acid for 48 h.