Example sentences for: serum-free

How can you use “serum-free” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Sixteen hours before the addition of drugs, the culture medium was removed and replaced by fresh serum-free DMEM.

  • Cerebella were dissected and processed as described elsewhere [ 61 ] . The dissociated cells were grown in a serum-free Neurobasal-A medium with B27 supplement (10 ml/500 ml) [ 62 ] , and in the presence of 25 mM KCl (Sigma).

  • This effect at higher doses might be due to the reported apoptotic effects of CDDO [ 25 26 ] . To examine for such effects, SW-1353 cells were treated for 24 hours with CDDO under serum-free conditions, and then IL-1 was added for a further 18 hours.

  • The growth medium was replaced with serum-free Ham's F12 for 36 h.

  • The DNA was mixed with 2 μl of Lipofectamine 2000 reagent (Life Technologies, Rockville, MD, USA) in a total volume of 50 μl of serum-free RPMI per cm 2of cells, incubated for 3 hours, and followed by the addition of an equal volume of media containing 20% fetal calf serum.


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