Example sentences for: serum-free

How can you use “serum-free” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Serum-starved cells were incubated with fresh serum-free media for 1 hour at 37°C (in order to return the phosphorylation levels of p44/42 MAPK to the basal state) and then treated with insulin (2.

  • Elegant studies in knockout mice have delineated the contribution of IGF-I, IGF-II and the IGF-I receptor to fetal growth [for review see ref [ 36 ] ]. Embryonic fibroblasts derived from IGF-IR knockout embryos (R-cells) do not grow in serum-free medium, despite supplementation with a wide variety of other growth factors, and grow more slowly in media containing serum than wild-type cells [for reviews see refs [ 37 38 ] ]. Additionally, all phases of the cell cycle are prolonged in the null cells suggesting that the IGF-IR is required throughout the cell cycle.

  • All experiments were performed after overnight equilibration with macrophage serum-free medium (GIBCO, San Diego, California, United States; Invitrogen, Carlsbad, California, United States) supplemented with 5 ng/ml GM-CSF.

  • This effect at higher doses might be due to the reported apoptotic effects of CDDO [ 25 26 ] . To examine for such effects, SW-1353 cells were treated for 24 hours with CDDO under serum-free conditions, and then IL-1 was added for a further 18 hours.

  • Fibroblasts at 70% confluence were incubated for 4 hrs in serum-free DMEM containing 4 μCi/ml methyl- 3H-thymidine (87 Ci/mmol) (NEN, Boston, MA).


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