Words similar to serum-free

Example sentences for: serum-free

How can you use “serum-free” in a sentence? Here are some example sentences to help you improve your vocabulary:

Trypsinization was subsequently blocked by exposure to 100% heat-inactivated fetal bovine serum (FBS; Harlan Bioproducts for Science, Inc., Indianapolis, IN) for 5 minutes and the tissue was washed 2 times with serum-free Ham's F12 medium.
Confluent MGBE were fed serum-free medium.
For determination of Gb3 content, cells grown in 6-well plates were placed in serum-free media for 24 hr followed by addition of the steroids for 1, 2 or 7 days.
The resulting pMIRB-HA-NPM3 plasmids (both sense and antisense orientations) were transiently transfected into NIH 3T3 cells, using transfection conditions with Lipofectamine and OptiMEM serum-free medium (Gibco-BRL, Bethesda, MD) as described [ 49 ] . Following a six-hour incubation of the DNA-Lipofectamine complexes in OptiMEM, the cells were washed and incubated in 10-cm dishes with their usual growth media (DMEM with 10% v/v fetal calf serum, 2 mM L-glutamine, 100 Units/ml of Penicillin G and 100 μg/ml Streptomycin) for 48-72 hours at 37°C in humidified 5% CO 2 incubators.
Due to the problem of cell death (>80%) in serum-free medium, cell cycle synchronization of the dermal fibroblasts in serum-free medium was not performed prior to 3H-thymidine assays.