Words similar to sertoli
Example sentences for: sertoli
How can you use “sertoli” in a sentence? Here are some example sentences to help you improve your vocabulary:
Androgen binding protein (ABP) is produced by Sertoli cells of the testis, secreted into the lumen of seminiferous tubules, and transported with tubular fluid to the epididymis, where it is internalized and degraded by the epithelial cells of the initial segment and the caput; it binds testosterone (T), dihydrotestosterone and estradiol with high affinity [ 9 10 11 ] . The functions of ABP have been the subject of many reviews [e.g.
For each reaction, 5 × 10 3cpm of labeled probe was incubated with 5 μg of crude nuclear proteins from Sertoli cells and 1.0 μg of poly dI:dC in a buffer containing 5 mM Hepes pH 7.9, 26 % glycerol, 0.2 mM EDTA, 0.5 mM DTT, 0.5 mM PMSF, 150 mM KCl and 5 mM MgCl at room temperature for 15 min.
, [ 10 12 13 14 15 ] ]. It is thought to regulate spermatogenesis and sperm maturation by maintaining high androgen levels in the testis and epididymis [ 16 17 ] and has been proposed as a biochemical marker for Sertoli cell function and formation of blood-testis barrier [ 12 18 ] . More recently, cell surface receptors for ABP and its liver-derived homolog sex hormone-binding globulin (SHBG) were identified in several androgen target tissues, suggesting that these extracellular steroid-binding proteins may have a much broader, hormone-like function [ 19 20 21 ] .
C/EBP is previously reported to be an important accessory factor for transactivation by several other transcription factors [ 26 ] . C/EBPβ was shown to transactivate the α1-acid glycoprotein gene promoter even when mutated in the DNA-binding domain, and this activation appeared to depend on protein-protein interaction with glucocorticoid receptor through the leucine zipper region [ 27 ] . Recently, a conserved domain in C/EBPα and C/EBPβ was shown to mediate cAMP responsiveness, strengthening the fact that C/EBP may be characterized as a cAMP-responsive nuclear regulator [ 28 29 ] . On the COX-2 promoter in granulosa cells, cAMP-mediated regulation was shown to be mediated primarily by an E-box that bind USF1 and USF2 isoforms in a manner resembling the RIIβ E-box in Sertoli cells [ 30 ] . Transactivation of the COX-2 gene in skin carcinoma cells is highly dependent on this E-box together with a C/EBP binding site that bind C/EBPβ and C/EBPδ [ 31 ] . These data strengthens a model of formation of a cAMP-responsive unit (CRU) containing USF and C/EBP.
20 μg of nuclear extract from Sertoli cell were loaded in 10% SDS PAGE gels and immunoblotting was performed as previously described [ 42 ] . Equal loading was determined by coomassie staining.
Loading...