Words similar to sertoli

Example sentences for: sertoli

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For each reaction, 5 × 10 3cpm of labeled probe was incubated with 5 μg of crude nuclear proteins from Sertoli cells and 1.0 μg of poly dI:dC in a buffer containing 5 mM Hepes pH 7.9, 26 % glycerol, 0.2 mM EDTA, 0.5 mM DTT, 0.5 mM PMSF, 150 mM KCl and 5 mM MgCl at room temperature for 15 min.
After 2 days of culture in serum free medium, lipofectamine-mediated transfections of primary cultures of rat Sertoli cells were performed (2 μg DNA per well in 6-well plates) and assayed as described by Grønning et al [ 17 ] .
20 μg of nuclear extract from Sertoli cell were loaded in 10% SDS PAGE gels and immunoblotting was performed as previously described [ 42 ] . Equal loading was determined by coomassie staining.
A small shift in mobility of this complex was observed in extracts prepared from Sertoli cells treated with 8-CPT-cAMP for 6 h (data not shown).
Dazl1 and Dazap1 transcripts were also present in the testes of W v/W vmutant mice which contained diminished number of germ cells [ 18 ] . However, only Dazap1 was expressed in a mouse germ cell line GCl-spg [ 19 ] and a Sertoli cell line MT4.