Words similar to sertoli
Example sentences for: sertoli
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For USF1, we observed that a new immunoreactive protein of about 31 kDa was formed only in nuclear extracts from cAMP-stimulated Sertoli cells (Fig.
The promoters of several genes induced by FSH in Sertoli cells and granulosa cells, including c-Fos, transferrin, follicle stimulating hormone receptor, SF-1 genes and the RIIβ gene, contain functional E-box elements [ 21 22 23 24 ] . We found that several USF complexes containing full-length USF1 and USF2 as well as truncated versions, are formed at the consensus E-box/HLH-binding element in the RIIβ promoter in Sertoli cell nuclear extracts.
Electrophoretic mobility shift assay (EMSA) with extracts from Sertoli cells demonstrated specific binding to the conserved element using the footprinted region as probe [ 20 ] . When incubated with proteins from Sertoli cell nuclear extracts at least five complexes were formed (Fig.
For each reaction, 5 × 10 3cpm of labeled probe was incubated with 5 μg of crude nuclear proteins from Sertoli cells and 1.0 μg of poly dI:dC in a buffer containing 5 mM Hepes pH 7.9, 26 % glycerol, 0.2 mM EDTA, 0.5 mM DTT, 0.5 mM PMSF, 150 mM KCl and 5 mM MgCl at room temperature for 15 min.
, [ 10 12 13 14 15 ] ]. It is thought to regulate spermatogenesis and sperm maturation by maintaining high androgen levels in the testis and epididymis [ 16 17 ] and has been proposed as a biochemical marker for Sertoli cell function and formation of blood-testis barrier [ 12 18 ] . More recently, cell surface receptors for ABP and its liver-derived homolog sex hormone-binding globulin (SHBG) were identified in several androgen target tissues, suggesting that these extracellular steroid-binding proteins may have a much broader, hormone-like function [ 19 20 21 ] .
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