Words similar to sertoli
Example sentences for: sertoli
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We have previously shown that C/EBPβ functions as a secondary effector of cAMP signaling on slow response genes in Sertoli cells, including RIIβ [ 17 ] . Both C/EBP and the RIIβ E-box is shown be involved in cAMP responsiveness of the RIIβ gene [ 17 18 ] .
After 2 days of culture in serum free medium, lipofectamine-mediated transfections of primary cultures of rat Sertoli cells were performed (2 μg DNA per well in 6-well plates) and assayed as described by Grønning et al [ 17 ] .
Among genes that are stimulated by FSH/cAMP during the period of maturation of Sertoli cells (15 to 20 days of age in rats), are several subunits of cAMP-dependent protein kinase (PKA), the serine/threonine kinase mainly responsible for the downstream effects of FSH [ 15 ] . Expression of the RIIβ regulatory subunit of cAMP-dependent protein kinase is highly induced (50-fold) at the mRNA level in primary cultures of rat Sertoli cells as a late response to cAMP peaking at 12 hours [ 16 ] . We have demonstrated that expression of CAAT/Enhancer binding protein β (C/EBPβ) is induced by cAMP with rapid kinetics in Sertoli cells, and that C/EBPβ is responsible for induction of late response genes like the RIIβ-gene [ 17 ] . The RIIβ promoter contains a conserved E-box/HLH element in the basal promoter localized at -280 to -275 relative to the functional ATG [ 18 ] . This element is shown to be important for cAMP-responsiveness in granulosa cells, and it has been shown to bind both USF and Myc in CHO and NB2a cells [ 18 19 ] . In this work, we show that USF isoforms may regulate cAMP responsiveness of the RIIβ promoter by modulating the effect of C/EBP, and that the formation of USF isoforms may be regulated by cAMP in Sertoli cells.
20 μg of nuclear extract from Sertoli cell were loaded in 10% SDS PAGE gels and immunoblotting was performed as previously described [ 42 ] . Equal loading was determined by coomassie staining.
USF isoforms are important for expression of several genes involved in Sertoli cell growth and differentiation.
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