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Example sentences for: sertoli
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USF isoforms are important for expression of several genes involved in Sertoli cell growth and differentiation.
Dazl1 and Dazap1 transcripts were also present in the testes of W v/W vmutant mice which contained diminished number of germ cells [ 18 ] . However, only Dazap1 was expressed in a mouse germ cell line GCl-spg [ 19 ] and a Sertoli cell line MT4.
1. USF1 and USF2, as well as truncated USF isoforms, bind to a consensus E-box in the RIIβ promoter in Sertoli cells.
Among genes that are stimulated by FSH/cAMP during the period of maturation of Sertoli cells (15 to 20 days of age in rats), are several subunits of cAMP-dependent protein kinase (PKA), the serine/threonine kinase mainly responsible for the downstream effects of FSH [ 15 ] . Expression of the RIIβ regulatory subunit of cAMP-dependent protein kinase is highly induced (50-fold) at the mRNA level in primary cultures of rat Sertoli cells as a late response to cAMP peaking at 12 hours [ 16 ] . We have demonstrated that expression of CAAT/Enhancer binding protein β (C/EBPβ) is induced by cAMP with rapid kinetics in Sertoli cells, and that C/EBPβ is responsible for induction of late response genes like the RIIβ-gene [ 17 ] . The RIIβ promoter contains a conserved E-box/HLH element in the basal promoter localized at -280 to -275 relative to the functional ATG [ 18 ] . This element is shown to be important for cAMP-responsiveness in granulosa cells, and it has been shown to bind both USF and Myc in CHO and NB2a cells [ 18 19 ] . In this work, we show that USF isoforms may regulate cAMP responsiveness of the RIIβ promoter by modulating the effect of C/EBP, and that the formation of USF isoforms may be regulated by cAMP in Sertoli cells.
For USF1, we observed that a new immunoreactive protein of about 31 kDa was formed only in nuclear extracts from cAMP-stimulated Sertoli cells (Fig.
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