Words similar to serum-containing
Example sentences for: serum-containing
How can you use “serum-containing” in a sentence? Here are some example sentences to help you improve your vocabulary:
To determine whether TGFβ1 could eliminate differences in α-SMA levels between PP and non-PP fibroblasts, cultures were incubated at confluence for three days with serum-free or serum-containing media supplemented with 0 ng/ml or 10 ng/ml active TGFβ1, and α-SMA and apoptosis were evaluated (Fig.
After 24 hours the cells were changed to 0.5% serum-containing medium, and Hh protein or compounds were added; 24 hours later the cells were either monitored for luciferase activity using the Luc-lite assay kit (Packard Instrument Company, Meriden, USA) or harvested for RNA isolation using an RNA isolation kit (Qiagen; Valencia, USA).
MCF-7 cells were grown in culture plates (tissue culture grade, 12 wells, flat bottom) in a final volume of 1 ml serum-containing culture medium per well, in a humidified atmosphere at 37°C and 5% CO 2 for 3 days.
The pig granulosa cell line PGC-2 was obtained from Bruce Downey (McGill University) and maintained in culture in McCoy's modified 5A medium supplemented with 10% FBS, as described previously in detail [ 19 ] . PGC-2 cells have been well-characterized with respect to steroidogenic capability, cAMP response, and gonadotropin insensitivity [ 19 ] . Here, PGC-2 were seeded at a density of approximately 5 × 10 5cells/ml of serum-containing medium in 24-well tissue culture plates.
When plasmids expressing the GR-GFP were injected into the nuclei of cells and the cells were grown in serum-containing medium, the majority of the expressed GR-GFP localized to the nuclei of the injected cells, as expected due to the presence of glucocorticoids in the serum.
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