Words similar to sequenase
Example sentences for: sequenase
How can you use “sequenase” in a sentence? Here are some example sentences to help you improve your vocabulary:
The human EST (T87026) and additional human clones obtained by PCR were sequenced manually using T7 Sequenase 2.0 DNA sequencing kit (Amersham, Piscataway, NJ) or using a BigDye terminator cycle sequencing kit on an ABI Prism 310 capillary sequencer (PE Applied Biosystems, Foster City, CA).
All transcripts with a different restriction pattern were sequenced (primer sequences available upon request) using the Thermo Sequenase fluorescent labelled primer cycle sequencing kit (Amersham Biosciences) and analysed on an ALF-express automated DNA sequencer (Amersham Biosciences).
Dideoxy sequencing was carried out using the Sequenase version 2.0 DNA sequencing kit (US Biochemical) and the T7 and M13 reverse sequencing primers.
R-PCR samples were generated from 50 ng restricted DNA using the Round A/ Round B protocol described in [ 9 12 ] . Briefly, Sequenase (Amersham) was used for two cycles of synthesis using a degenerate primer (5'-GTTTCCCAGTCACGATCNNNNNNNNN-3' ('round A').
All DNA sequencing reactions were done using the dideoxy chain-termination protocol and T7 Sequenase v2.