Example sentences for: sequenase

How can you use “sequenase” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • PCR products were purified (GENECLEAN III, BIO 101 Inc.) to remove primers and other reagents, and sequenced (Thermo Sequenase ®, Amersham Life Science, Inc.).

  • The amplified DNA was cloned into the TA cloning vector pCRII (Invitrogen, Carlsbad, CA) and then sequenced by the Sanger method using the Sequenase (Amersham Life Science, Cleveland, OH) protocol as described previously [ 37].

  • All transcripts with a different restriction pattern were sequenced (primer sequences available upon request) using the Thermo Sequenase fluorescent labelled primer cycle sequencing kit (Amersham Biosciences) and analysed on an ALF-express automated DNA sequencer (Amersham Biosciences).

  • The single nucleotide polymorphisms used to determine M6P/IGF2R loss of heterozygosity were assessed by direct sequencing of PCR products according to the manufacturer's protocol (Thermo Sequenase, USB Corporation, Cleveland, OH) (Figure 1).

  • This step is used to verify - by sequencing with the Thermo Sequenase Radiolabeled Termintor Cycle Sequencing Kit (USB) - the composition of a particular band, which can be cut out, eluted and reamplified (not shown), or simply cloned from the P1-7A reaction (see Fig.


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