Example sentences for: sequenase

How can you use “sequenase” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • All transcripts with a different restriction pattern were sequenced (primer sequences available upon request) using the Thermo Sequenase fluorescent labelled primer cycle sequencing kit (Amersham Biosciences) and analysed on an ALF-express automated DNA sequencer (Amersham Biosciences).

  • Dideoxy sequencing was carried out using the Sequenase version 2.0 DNA sequencing kit (US Biochemical) and the T7 and M13 reverse sequencing primers.

  • The single nucleotide polymorphisms used to determine M6P/IGF2R loss of heterozygosity were assessed by direct sequencing of PCR products according to the manufacturer's protocol (Thermo Sequenase, USB Corporation, Cleveland, OH) (Figure 1).

  • The human EST (T87026) and additional human clones obtained by PCR were sequenced manually using T7 Sequenase 2.0 DNA sequencing kit (Amersham, Piscataway, NJ) or using a BigDye terminator cycle sequencing kit on an ABI Prism 310 capillary sequencer (PE Applied Biosystems, Foster City, CA).

  • This step is used to verify - by sequencing with the Thermo Sequenase Radiolabeled Termintor Cycle Sequencing Kit (USB) - the composition of a particular band, which can be cut out, eluted and reamplified (not shown), or simply cloned from the P1-7A reaction (see Fig.


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