Example sentences for: sequenase

How can you use “sequenase” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The human EST (T87026) and additional human clones obtained by PCR were sequenced manually using T7 Sequenase 2.0 DNA sequencing kit (Amersham, Piscataway, NJ) or using a BigDye terminator cycle sequencing kit on an ABI Prism 310 capillary sequencer (PE Applied Biosystems, Foster City, CA).

  • All transcripts with a different restriction pattern were sequenced (primer sequences available upon request) using the Thermo Sequenase fluorescent labelled primer cycle sequencing kit (Amersham Biosciences) and analysed on an ALF-express automated DNA sequencer (Amersham Biosciences).

  • Dideoxy sequencing was carried out using the Sequenase version 2.0 DNA sequencing kit (US Biochemical) and the T7 and M13 reverse sequencing primers.

  • R-PCR samples were generated from 50 ng restricted DNA using the Round A/ Round B protocol described in [ 9 12 ] . Briefly, Sequenase (Amersham) was used for two cycles of synthesis using a degenerate primer (5'-GTTTCCCAGTCACGATCNNNNNNNNN-3' ('round A').

  • All DNA sequencing reactions were done using the dideoxy chain-termination protocol and T7 Sequenase v2.


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