Words similar to sequenase

Example sentences for: sequenase

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The single nucleotide polymorphisms used to determine M6P/IGF2R loss of heterozygosity were assessed by direct sequencing of PCR products according to the manufacturer's protocol (Thermo Sequenase, USB Corporation, Cleveland, OH) (Figure 1).
PCR products were purified (GENECLEAN III, BIO 101 Inc.) to remove primers and other reagents, and sequenced (Thermo Sequenase ®, Amersham Life Science, Inc.).
The human EST (T87026) and additional human clones obtained by PCR were sequenced manually using T7 Sequenase 2.0 DNA sequencing kit (Amersham, Piscataway, NJ) or using a BigDye terminator cycle sequencing kit on an ABI Prism 310 capillary sequencer (PE Applied Biosystems, Foster City, CA).
R-PCR samples were generated from 50 ng restricted DNA using the Round A/ Round B protocol described in [ 9 12 ] . Briefly, Sequenase (Amersham) was used for two cycles of synthesis using a degenerate primer (5'-GTTTCCCAGTCACGATCNNNNNNNNN-3' ('round A').
Dideoxy sequencing was carried out using the Sequenase version 2.0 DNA sequencing kit (US Biochemical) and the T7 and M13 reverse sequencing primers.