Words similar to sepharose

Example sentences for: sepharose

How can you use “sepharose” in a sentence? Here are some example sentences to help you improve your vocabulary:

The 1 sttype III repeat of fibronectin was expressed as a fusion protein coupled to GST, and this GST-3FN1 fusion protein was coupled to Sepharose and used in affinity purification.
Insulin receptor was immunoprecipitated with specific antibodies, collected with protein A sepharose, and incubated with polyGlu 4 Tyr 1 peptides (Sigma, St. Louis, MO) in the presence of [γ- 32P]-ATP [ 14 ] . After completion of the kinase reaction, the mix was centrifuged and the phospho-peptide-containing supernatant precipitated with 20% TCA (w/v).
To ensure that arginine-Sepharose chromatography did not preferentially fractionate specific tubulin isotypes, microtubule protein fractions from an early stage in purification (tubulin plus MAPs) were examined by ELISAs and Western blotting.
One way in which integrins were originally shown to be fibronectin receptors was by affinity chromatography of radioiodinated cell surface proteins on a fibronectin column [ 27 28 ] . Consistent with these earlier studies, cell surface radioiodination of RASMCs followed by affinity chromatography on fibronectin Sepharose and elution with EDTA revealed the binding of two major bands at 120 kDa and 140 kDa (Fig.
10, most of the 46 kDa and 70 kDa HSPGs bound to III1-C Sepharose but not to III 11-C Sepharose.