Example sentences for: sepharose

How can you use “sepharose” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The supernatant was then loaded on a 2 ml arginine-Sepharose column equilibrated with the MES buffer above without Triton X-100.

  • The antibodies were affinity purified against the immunizing peptide (anti-TGF-β 3 ) or against protein A sepharose (anti-TGF-β 1 -LC, anti-TGF-β 1 -CC and anti-TGF-β 2 ). Immunohistochemical staining was performed using an indirect immunoperoxidase detection protocol (Vectastain Elite kit, Vector Laboratories, Burlingame, CA, USA).

  • The 1 sttype III repeat of fibronectin was expressed as a fusion protein coupled to GST, and this GST-3FN1 fusion protein was coupled to Sepharose and used in affinity purification.

  • 10, most of the 46 kDa and 70 kDa HSPGs bound to III1-C Sepharose but not to III 11-C Sepharose.

  • Lysates from induced LLWO2F cells were combined with GST-alone- or GST-PNUTS-loaded glutathione Sepharose beads.


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