Example sentences for: sepharose

How can you use “sepharose” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • They were then applied on a heparin-Sepharose column (bed volume 5 ml) that was equilibrated and run (flow rate 20 ml/h) with a buffer consisted of 50 mM sodium acetate, pH 5.6, containing 0.25 M NaCl and 0.01% Tween-20.

  • Briefly, gelatin-conjugated sepharose bead columns were pre-washed with PBS containing 2 mM EDTA and 0.1 mM PMSF.

  • Insulin receptor was immunoprecipitated with specific antibodies, collected with protein A sepharose, and incubated with polyGlu 4 Tyr 1 peptides (Sigma, St. Louis, MO) in the presence of [γ- 32P]-ATP [ 14 ] . After completion of the kinase reaction, the mix was centrifuged and the phospho-peptide-containing supernatant precipitated with 20% TCA (w/v).

  • Solubilized B. mori BBMV proteins were separated by Q Sepharose anion-exchange chromatography and all eluted fractions were tested for APN enzymatic activity.

  • Furthermore, when this wtPAI-1 was chromatographed on heparin-Sepharose it was found to be > 98% pure by silver staining of the gel (Fig.


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