Words similar to sepharose

Example sentences for: sepharose

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The 1 sttype III repeat of fibronectin was expressed as a fusion protein coupled to GST, and this GST-3FN1 fusion protein was coupled to Sepharose and used in affinity purification.
The antibodies were affinity purified against the immunizing peptide (anti-TGF-β 3 ) or against protein A sepharose (anti-TGF-β 1 -LC, anti-TGF-β 1 -CC, and anti-TGF-β 2 ), and have been assayed for specificity by Western blot analysis [ 19, 20, 33].
Complexes were recovered with Protein A Sepharose, washed twice with ice cold NP40 wash buffer, washed twice with ice cold Rlk kinase buffer (30 mM HEPES pH 7.4, 150 mM NaCl, 5 mM MgCl
The procedure was a modification of the procedure used to isolate the fibronectin receptor and other integrins [ 27 28 ] . The affinity resins were prepared by coupling fibronectin, III1-C or III 11-C to CNBr-activated Sepharose CL-4B according to the manufacturer's recommendations.
Briefly, equal nuclear cell extract (200 μg/sample) was mixed with 3 μg anti-E2F-4 antibodies at 4°C followed by the addition of 50 μl protein A-sepharose beads (Amersham Biotechnology).