Words similar to sepharose

Example sentences for: sepharose

How can you use “sepharose” in a sentence? Here are some example sentences to help you improve your vocabulary:

The 1 sttype III repeat of fibronectin was expressed as a fusion protein coupled to GST, and this GST-3FN1 fusion protein was coupled to Sepharose and used in affinity purification.
The sample was then loaded on a Q Sepharose HR 10/30 anion-exchange column.
Insulin receptor was immunoprecipitated with specific antibodies, collected with protein A sepharose, and incubated with polyGlu 4 Tyr 1 peptides (Sigma, St. Louis, MO) in the presence of [γ- 32P]-ATP [ 14 ] . After completion of the kinase reaction, the mix was centrifuged and the phospho-peptide-containing supernatant precipitated with 20% TCA (w/v).
Remaining proteins were then re-precipitated from the eluate using mAb directly conjugated to sepharose 4B beads for 30 min at 4°C, washed three times with 1% Triton X100 in PBS, eluted with Laemmli sample buffer, and resolved on SDS-PAGE.
The procedure for arginine-Sepharose purification of tubulin was presented in detail by Lacey and Snowdon [ 42 ] . An enriched tubulin fraction was collected in high-salt MES buffer (2.