Words similar to sepharose
Example sentences for: sepharose
How can you use “sepharose” in a sentence? Here are some example sentences to help you improve your vocabulary:
10, most of the 46 kDa and 70 kDa HSPGs bound to III1-C Sepharose but not to III 11-C Sepharose.
RASMC lysates were subjected to partial heparitinase treatment (full digestion was not performed in order to allow some GAGs to remain associated with the core proteins) then applied to either III1-C or III 11-C Sepharose.
The antibodies were affinity purified against the immunizing peptide (anti-TGF-β 3 ) or against protein A sepharose (anti-TGF-β 1 -LC, anti-TGF-β 1 -CC and anti-TGF-β 2 ). Immunohistochemical staining was performed using an indirect immunoperoxidase detection protocol (Vectastain Elite kit, Vector Laboratories, Burlingame, CA, USA).
They were then applied on a heparin-Sepharose column (bed volume 5 ml) that was equilibrated and run (flow rate 20 ml/h) with a buffer consisted of 50 mM sodium acetate, pH 5.6, containing 0.25 M NaCl and 0.01% Tween-20.
Cell lysates (prepared as described above) were incubated with glutathione sepharose beads for 2 hours at 4°C.
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