Words similar to sephadex
Example sentences for: sephadex
How can you use “sephadex” in a sentence? Here are some example sentences to help you improve your vocabulary:
The pellet was resuspended in 10 ml of buffer B (20 mM HEPES, pH 8.5, 1 mM EDTA, 1 mM DTT), desalted on a Sephadex G-25 column equilibrated with buffer B, then subjected to sequential chromatography on a 5 ml HiTrap heparin column (Pharmacia) and then a 5 ml HiTrap Q column (Pharmacia).
The reaction products were purified by means of three repeated gel chromatographies using water saturated Sephadex G-50 in Millipore Multiscreen ®filtration plates according to the instructions provided by the supplier and dried under vacuum.
After a 30 min incubation at 37°C, labeled probes were purified on Sephadex G50 spin columns.
After elution from the gel slice, the labeled single-stranded oligonucleotides were desalted by centrifugation through a Sephadex G10 spin column.
The molecular species of the total serum iCT in the serum was determined by radioimmunoassay of fractions obtained from Sephadex gel filtration of pooled hamster sera as described above.
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