Example sentences for: sephadex

How can you use “sephadex” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • . The probe was separated from the free nucleotides in a Sephadex G-50 column equilibrated with 50 mM tris(hydroxymethyl) aminomethane pH 8.0, 15 mM NaCl, and 1 mM EDTA.

  • The reaction products were purified by means of three repeated gel chromatographies using water saturated Sephadex G-50 in Millipore Multiscreen ®filtration plates according to the instructions provided by the supplier and dried under vacuum.

  • The supernatant fraction was collected and passed through a 1.5 × 5.5 cm DG-10 Sephadex column (Bio-Rad).

  • Purified compounds were obtained by Sephadex A-25 ion-exchange chromatography using a linear gradient of ammonium bicarbonate buffer (pH 7.5) as eluent, and their physicochemical characteristics are given in Table 3.

  • Unincorporated nucleotides were removed by size-exclusion chromatography on RNase-free Sephadex G-50 (Sigma) spin columns.


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