Words similar to sephadex
Example sentences for: sephadex
How can you use “sephadex” in a sentence? Here are some example sentences to help you improve your vocabulary:
Unincorporated nucleotides were removed by size-exclusion chromatography on RNase-free Sephadex G-50 (Sigma) spin columns.
To eliminate the lyophilization step from the protocol, we used an alternative column-the Sephadex™ G-50 column-to filter out free nucleotides from the ds cDNA after completion of the second strand synthesis reaction.
Sequencing reactions were processed through 96-well Sephadex G-50 SF plates (Multiscreen filter plates; Millipore) and loaded onto ABI Prism 3700 DNA Analyzer.
The reaction products were purified by means of three repeated gel chromatographies using water saturated Sephadex G-50 in Millipore Multiscreen ®filtration plates according to the instructions provided by the supplier and dried under vacuum.
The pellet was resuspended in 10 ml of buffer B (20 mM HEPES, pH 8.5, 1 mM EDTA, 1 mM DTT), desalted on a Sephadex G-25 column equilibrated with buffer B, then subjected to sequential chromatography on a 5 ml HiTrap heparin column (Pharmacia) and then a 5 ml HiTrap Q column (Pharmacia).
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