Example sentences for: sephadex

How can you use “sephadex” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Similarly to work previously reported [ 20], constituted extracts, in 1-10 ml 0.2% gelatin or 0.2% HSA, were applied to calibrated 2.5 × 100 cm columns containing G-75 superfine Sephadex (Pharmacia Biotech, Piscataway, New Jersey, USA) suspended in 0.1% human serum albumin (1 g HSA, 0.1 mol NH 4 HCO 3 and 0.1 g merthiolate/l at pH = 7.5) at 4°C.

  • The supernatant fraction was collected and passed through a 1.5 × 5.5 cm DG-10 Sephadex column (Bio-Rad).

  • Sequencing reactions were processed through 96-well Sephadex G-50 SF plates (Multiscreen filter plates; Millipore) and loaded onto ABI Prism 3700 DNA Analyzer.

  • Sephadex G-50 was from Pharmacia.

  • To eliminate the lyophilization step from the protocol, we used an alternative column-the Sephadex™ G-50 column-to filter out free nucleotides from the ds cDNA after completion of the second strand synthesis reaction.


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