Words similar to sephadex

Example sentences for: sephadex

How can you use “sephadex” in a sentence? Here are some example sentences to help you improve your vocabulary:

Similarly to work previously reported [ 20], constituted extracts, in 1-10 ml 0.2% gelatin or 0.2% HSA, were applied to calibrated 2.5 × 100 cm columns containing G-75 superfine Sephadex (Pharmacia Biotech, Piscataway, New Jersey, USA) suspended in 0.1% human serum albumin (1 g HSA, 0.1 mol NH 4 HCO 3 and 0.1 g merthiolate/l at pH = 7.5) at 4°C.
The reaction products were purified by means of three repeated gel chromatographies using water saturated Sephadex G-50 in Millipore Multiscreen ®filtration plates according to the instructions provided by the supplier and dried under vacuum.
The pellets from the ammonium sulfate fractionation were resuspended in 60 ml of buffer B (20 mM HEPES pH 8.5 4°C, 1 mM EDTA, and 0.1% 2-mercaptoethanol), desalted on a G-25 sephadex column equilibrated with buffer B, then subjected to sequential chromatography on a 5 ml HiTrap Heparin column (Pharmacia), a 5 ml HiTrap Q column (Pharmacia) and a HiTrap SP column (Pharmacia) respectively.
Sephadex G-50 was from Pharmacia.
Purified compounds were obtained by Sephadex A-25 ion-exchange chromatography using a linear gradient of ammonium bicarbonate buffer (pH 7.5) as eluent, and their physicochemical characteristics are given in Table 3.