Example sentences for: sephadex

How can you use “sephadex” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • To eliminate the lyophilization step from the protocol, we used an alternative column-the Sephadex™ G-50 column-to filter out free nucleotides from the ds cDNA after completion of the second strand synthesis reaction.

  • To examine whether mactinin was present in immune complexes in rheumatoid arthritis fluid, some samples were acidified by dialysis against 0.1 M sodium acetate, pH 4.1, to dissociate complexes [ 18 ] . The acidified samples were first fractionated on an 800 ml G-75 Sephadex (Pharmacia, Piscataway, NJ) size-exclusion column.

  • Sephadex G-50 was from Pharmacia.

  • The supernatant fraction was collected and passed through a 1.5 × 5.5 cm DG-10 Sephadex column (Bio-Rad).

  • Time-resolved UV/vis spectroscopy methods were as described previously [ 18 ] . A Phenylsepharose™-purified pR sample was reconstituted into mixed micelles containing 1,2-diheptanoyl-SN-glycero-3-phosphocholine (DHPC), by adding a 1% solution of the short-chain lipid and then removing most of the detergent on a Sephadex G-25 column equilibrated with 1% DHPC in 100 mM NaCl.


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