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Example sentences for: rpmi-

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7 murine macrophages (American Type Culture Collection, Rockville, MD) were cultured at 37°C in a humidified incubator (95 % air with 5% CO 2 ) in RPMI-1640 medium with 10 % fetal bovine serum (FBS), 50 units/ml penicillin, and 50 mg/ml streptomycin (culture medium).
Dexamethasone, ascorbic acid-2 phosphate, β-glycerophosphate, bovine serum albumin (BSA), and FCS were purchased from Sigma Diagnostics (St Louis, MO, USA); penicillin, streptomycin, DMEM, and RPMI-1640 from Biowhittaker (Watersville, MD, USA); Lymphoprep ™from Nycomed, Oslo, Norway.
Medium consisted of RPMI-1640 + 10% fetal bovine serum and antibiotics.
Cells (in supplemented RPMI-1640 medium containing 0.1% serum) were then incubated for 48 h in the continued presence of gefitinib or erlotinib.
Following incubation with gefitinib or erlotinib, monolayers were washed twice with PBS (containing calcium and magnesium) and incubated with 7.5 μmol Calcein AM in supplemented RPMI-1640 (no serum) for 30 min.