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Example sentences for: rpmi-
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Both HL60/TLK286 and HL60 cell lines were cultured in RPMI-1640 medium supplemented with 2 mM L-glutamine, 50 U/ml penicillin, 50 μg/ml streptomycin and 10% heat-inactivated fetal bovine serum.
HIV-1 IIIB (100 TCID 50 ) in the presence or absence of graded concentrations of virus inhibitors, CAP and sCD4, respectively, in RPMI-1640 medium containing 10% fetal bovine serum (FBS) were mixed with MT-2 cells (10 4cells/well) and placed into 96-well polystyrene plates.
4 × 10 8virus particles) (unlike in the experiments described in an earlier report [ 8 ] where infectious tissue culture medium was used instead), were serially diluted 2- to 256-fold in RPMI-1640 medium containing 10% FBS, mixed with MT-2 cells and placed into 96-well polystyrene plates.
Ficoll hypaque, RPMI-1640 and all other culture reagents were purchased from Sigma Chemical Co., St. Louis, U.S.A. 51Chromium was obtained from BARC, Mumbai, India.
Following overnight incubation, cells were grown for 24 h in the supplemented RPMI-1640 medium with 0.1% serum.