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Example sentences for: rpmi-

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All cells were cultured at 37°C up to 10 5cells per ml in RPMI-1640 media, containing 10% Fetal Bovine Serum (FBS) treated with a mixture of 1% streptomycin and penicillin antibiotics, and 1% L-glutamine (Gibco/BRL).
Fetal calf serum and RPMI-1640 culture medium supplemented with 25 mM HEPES were from Irvine Scientific.
Ficoll hypaque, RPMI-1640 and all other culture reagents were purchased from Sigma Chemical Co., St. Louis, U.S.A. 51Chromium was obtained from BARC, Mumbai, India.
7 murine macrophages (American Type Culture Collection, Rockville, MD) were cultured at 37°C in a humidified incubator (95 % air with 5% CO 2 ) in RPMI-1640 medium with 10 % fetal bovine serum (FBS), 50 units/ml penicillin, and 50 mg/ml streptomycin (culture medium).
These cells were cultured in RPMI-1640 medium supplemented with 10% FCS and 0.05 mM 2-mercaptoethanol (minus antibiotic/ antimycotic).