Words similar to rpmi

Example sentences for: rpmi

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The washed PBMC were suspended in complete RPMI medium and adjusted to a volume of 10 × 10 6cells/ml.
A weakly immunogenic lung cancer, Lewis lung carcinoma (3LL, H-2 b) obtained from ATCC (CRL-1642, LLC) was utilized for assessment of cytokines important for SLC/CCL21 - mediated anti-tumor responses in vivo . The cells were routinely cultured as monolayers in 25 cm 3tissue culture flasks containing RPMI 1640 medium (Irvine Scientific, Santa Anna, CA) supplemented with 10% fetal bovine serum (FBS) (Gemini Bioproducts, Calabasas, CA), penicillin (100 U/ml), streptomycin (0.
THP-1 cells were grown in RPMI 1640 media (GIBCO BRL) containing 10% fetal calf serum (FCS) (GIBCO BRL) at 37°C in 5% CO 2 [ 21 ] . To differentiate the THP-1 cells into macrophage-like cells, the cells were treated with 10 μM phorbol myristate acetate (PMA) (Sigma) as follows [ 21 ] . All PMA manipulations were done under low light conditions.
The electroporated cells were diluted in 3 ml of complete RPMI 1640 medium and maintained at 37°C for 36-48 hours.
P3HR-1 (ATCC) and BJAB-B1 cells (generously donated by Dr. George Miller) are EBV latently infected B-cells kept in RPMI 1640 medium (supplemented with 10% FBS and an antibiotic-mycotic solution) (Gibco, Invitrogen Corp., Carlsbad, CA).