Example sentences for: rpmi

How can you use “rpmi” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The cell suspension was brought to 10 ml with cold RPMI media, and the desired T cell subset was obtained by magnetic removal of the contaminating cells.

  • 10 6adherent mononuclear cells/ml were incubated in a 24 well culture plate in complete RPMI and supernatant was evaluated at 24 hr for basal production of IL10 by ELISA (R&D).

  • Jurkat cells expressing SV40 large T antigen (Jurkat Tag) were a generous gift from Dr. Gerald Crabtree (Stanford) and were maintained in RPMI 1640 supplemented with 10% fetal calf serum, penicillin, streptomycin, glutamine, and 50 μM 2-mercaptoethanol.

  • RPMI 1640, penicillin, streptomycin, fungizone, and trypsin/EDTA were obtained from Cellgro (Herndon, VA, USA).

  • A weakly immunogenic lung cancer, Lewis lung carcinoma (3LL, H-2 b) obtained from ATCC (CRL-1642, LLC) was utilized for assessment of cytokines important for SLC/CCL21 - mediated anti-tumor responses in vivo . The cells were routinely cultured as monolayers in 25 cm 3tissue culture flasks containing RPMI 1640 medium (Irvine Scientific, Santa Anna, CA) supplemented with 10% fetal bovine serum (FBS) (Gemini Bioproducts, Calabasas, CA), penicillin (100 U/ml), streptomycin (0.


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