Example sentences for: rpmi

How can you use “rpmi” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • These cells were cultured in RPMI medium supplemented with 10% FCS, 2 mM L-glutamine, and 0.05 mM 2-mercaptoethanol without antibiotic/antimycotic.

  • THP-1 cells were harvested by centrifugation for 10 minutes at 228 × g , and the pellet was resuspended in RPMI 1640/10% FCS/10 μM PMA to give a cell density of approximately 1 × 10 6THP-1 cells/mL.

  • Each well was then washed twice with 3 mL of RPMI 1640/10% FCS, and then 1 mL of 0.1% (v/v) Triton X-100 (Sigma) was added to each well to lyse the macrophages.

  • The synovial tissue was minced and incubated with 0.3 mg/ml collagenase (Sigma, St Louis, MO, USA) for 1h at 37°C in RPMI 1640 medium (Life Technologies, Gaithersburg, MD, USA).

  • Human prostate carcinoma cells, LNCaP (ATCC, Rockville, MD), were maintained and propagated in T25 flasks in RPMI 1640 media (GIBCO-BRL, Gaithersburg, MD) supplemented with 10% FCS (Gemini Bio-Products, Calabasas, CA), 2 mM glutamine, Fungizone (1 μg/ml) and penicillin (200 unit/ml)/ streptomycin (200 μg/ml) (referred as complete medium) in a 37°C humidified 5% CO 2 incubator.


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