Words similar to ripa

Example sentences for: ripa

How can you use “ripa” in a sentence? Here are some example sentences to help you improve your vocabulary:

The whole cell extracts were prepared and used for Western blot with anti-PDGF receptor α using RIPA buffer containing SDS.
Frozen lung tissue was homogenized in ice cold 750 μl radioimmunoprecipitation assay (RIPA) buffer (1×PBS, 1% Igepal CA-630, 0.5% sodium deoxycholate, 0.1% SDS) plus protease inhibitors (30 μl/ml RIPA aprotinin, 10 μl/ml RIPA 100 mmol/l sodium orthovanadate).
The cell pellets were suspended in 150-300 μl ice-cold RIPA lysis buffer (1% NP-40, 0.5% Na-deoxycholate, 0.1% SDS in PBS, 0.5 mM Phenylmethylsulfonyl fluoride (PMSF), 0.02 mg/ml Aprotinin, 1 mM Na 3 VO 4 ) and incubated for 30 min on ice.
Samples were triturated two to three times through 21-gauge then 25-gauge needles, and 10 μl/ml RIPA 10 mg/ml phenyl methylsulfonyl fluoride was added.
Phospho-tyrosine phosphatase assays were performed similarly to previously described protocols [ 14 58 ] . For the preparation of tyrosine-phosphorylated substrate, 7 × 10 6HTC-IR cells [ 59 ] were incubated with medium containing insulin (50 nM/ml) and lysed with RIPA buffer.