Words similar to ripa

Example sentences for: ripa

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Samples of renal cortex from S and SD rats on the 0.3% and 8.0% NaCl diets for 7 and 21 days were diced into small pieces and chilled in 3 ml of ice cold RIPA buffer per gram of tissue, then homogenized (Omni-Mixer 17105; Omni, Waterbury, CT) in standard fashion.
Cells were solubilized in RIPA buffer (50 mM Tris-HCl [pH8.
protocol to study subconfluent podocyte growth and podocyte growth during establishment of mature cell-cell contacts between differentiated podocytes [ 27 28 ] . Briefly, RNA and protein were extracted from parallel plates of podocytes using TRIZOL Reagent or RIPA buffer, respectively, under three conditions: 1) during sub-confluent growth at 33°C; 2) immediately upon reaching confluency (day zero); and 3) on day 14 after switching confluent cultures to 37°C.
Phospho-tyrosine phosphatase assays were performed similarly to previously described protocols [ 14 58 ] . For the preparation of tyrosine-phosphorylated substrate, 7 × 10 6HTC-IR cells [ 59 ] were incubated with medium containing insulin (50 nM/ml) and lysed with RIPA buffer.
Samples were triturated two to three times through 21-gauge then 25-gauge needles, and 10 μl/ml RIPA 10 mg/ml phenyl methylsulfonyl fluoride was added.