Words similar to ripa

Example sentences for: ripa

How can you use “ripa” in a sentence? Here are some example sentences to help you improve your vocabulary:

The cell pellets were suspended in 150-300 μl ice-cold RIPA lysis buffer (1% NP-40, 0.5% Na-deoxycholate, 0.1% SDS in PBS, 0.5 mM Phenylmethylsulfonyl fluoride (PMSF), 0.02 mg/ml Aprotinin, 1 mM Na 3 VO 4 ) and incubated for 30 min on ice.
Total cell lysates were prepared by lysis of cells with RIPA buffer [ 63 ] . Thirty μg of each sample was processed by Western blot analysis as described [ 31 ] . Antibodies against cell cycle-regulatory proteins as well as those against focal adhesion kinase were purchased from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA).
Samples were triturated two to three times through 21-gauge then 25-gauge needles, and 10 μl/ml RIPA 10 mg/ml phenyl methylsulfonyl fluoride was added.
Cells were solubilized in RIPA buffer (50 mM Tris-HCl [pH8.
Phospho-tyrosine phosphatase assays were performed similarly to previously described protocols [ 14 58 ] . For the preparation of tyrosine-phosphorylated substrate, 7 × 10 6HTC-IR cells [ 59 ] were incubated with medium containing insulin (50 nM/ml) and lysed with RIPA buffer.