Words similar to ripa

Example sentences for: ripa

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Cells were solubilized in RIPA buffer (50 mM Tris-HCl [pH8.
Phospho-tyrosine phosphatase assays were performed similarly to previously described protocols [ 14 58 ] . For the preparation of tyrosine-phosphorylated substrate, 7 × 10 6HTC-IR cells [ 59 ] were incubated with medium containing insulin (50 nM/ml) and lysed with RIPA buffer.
protocol to study subconfluent podocyte growth and podocyte growth during establishment of mature cell-cell contacts between differentiated podocytes [ 27 28 ] . Briefly, RNA and protein were extracted from parallel plates of podocytes using TRIZOL Reagent or RIPA buffer, respectively, under three conditions: 1) during sub-confluent growth at 33°C; 2) immediately upon reaching confluency (day zero); and 3) on day 14 after switching confluent cultures to 37°C.
eNOS = endothelial nitric oxide synthase; iNOS = inducible nitric oxide synthase; nNOS = neuronal nitric oxide synthase; NO = nitric oxide; NOS = nitric oxide synthase; PBS = phosphate-buffered saline; PCR = polymerase chain reaction; RIPA = radioimmunoprecipitation assay; RVsP = right ventricular systolic pressure.
Frozen lung tissue was homogenized in ice cold 750 μl radioimmunoprecipitation assay (RIPA) buffer (1×PBS, 1% Igepal CA-630, 0.5% sodium deoxycholate, 0.1% SDS) plus protease inhibitors (30 μl/ml RIPA aprotinin, 10 μl/ml RIPA 100 mmol/l sodium orthovanadate).