Words similar to ripa
Example sentences for: ripa
How can you use “ripa” in a sentence? Here are some example sentences to help you improve your vocabulary:
Five hundred microliters of supplemented RIPA lysis buffer (50 mM Tris-HCI pH 7.5, 150 mM NaCI, 2 mM EGTA, 0.1% Triton X-100, 1 mM PMSF, 10 ug/ml aprotinin, 10 ug/ml leupeptin, 1 mM sodium orthovanadate) were added to each dish and cells were harvested with a rubber policeman.
Samples were triturated two to three times through 21-gauge then 25-gauge needles, and 10 μl/ml RIPA 10 mg/ml phenyl methylsulfonyl fluoride was added.
RIPA, radioimmunoprecipitation assay
Total cell lysates were prepared by lysis of cells with RIPA buffer [ 63 ] . Thirty μg of each sample was processed by Western blot analysis as described [ 31 ] . Antibodies against cell cycle-regulatory proteins as well as those against focal adhesion kinase were purchased from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA).
Cells were solubilized in RIPA buffer (50 mM Tris-HCl [pH8.
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