Words similar to riiβ
Example sentences for: riiβ
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An inhibitory effect of USF on Myc-mediated cellular proliferation has been demonstrated in HeLa cells [ 33 ] , and a non-canonical HLH element inhibits expression of the fibroblast growth factor binding protein (FGF-BP) in several carcinoma cells [ 34 ] . Furthermore, USF2 inhibits binding of hypoxia-inducible factor-1 (HIF-1) to the plasminogen activator inhibitor -1 (PAI-1) gene in hepatocytes in order to disturb transcriptional activation [ 35 ] . Our results add C/EBPβ-mediated activation of RIIβ to the mechanisms inhibited by USF.
We have previously shown that C/EBPβ functions as a secondary effector of cAMP signaling on slow response genes in Sertoli cells, including RIIβ [ 17 ] . Both C/EBP and the RIIβ E-box is shown be involved in cAMP responsiveness of the RIIβ gene [ 17 18 ] .
C/EBP is previously reported to be an important accessory factor for transactivation by several other transcription factors [ 26 ] . C/EBPβ was shown to transactivate the α1-acid glycoprotein gene promoter even when mutated in the DNA-binding domain, and this activation appeared to depend on protein-protein interaction with glucocorticoid receptor through the leucine zipper region [ 27 ] . Recently, a conserved domain in C/EBPα and C/EBPβ was shown to mediate cAMP responsiveness, strengthening the fact that C/EBP may be characterized as a cAMP-responsive nuclear regulator [ 28 29 ] . On the COX-2 promoter in granulosa cells, cAMP-mediated regulation was shown to be mediated primarily by an E-box that bind USF1 and USF2 isoforms in a manner resembling the RIIβ E-box in Sertoli cells [ 30 ] . Transactivation of the COX-2 gene in skin carcinoma cells is highly dependent on this E-box together with a C/EBP binding site that bind C/EBPβ and C/EBPδ [ 31 ] . These data strengthens a model of formation of a cAMP-responsive unit (CRU) containing USF and C/EBP.
Electrophoretic mobility shift assays (EMSAs) were performed using a double stranded [ 32P]-end-labeled oligonucleotide covering the consensus E-box/HLH element (underlined) and flanking sequences from the RIIβ promoter (-305, 5'-GATCGCCGGGTTGCCATGGTTT CCGGGGAT CACGTGGGCGCGCGG-3', -268).
As shown in figure 4, RIIβ is found in the postsynaptic region of the NMJ and did not precisely overlap with the α-bungarotoxin label but instead encompassed a broader area of this region.
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