Example sentences for: reverse-transcription

How can you use “reverse-transcription” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • 5-5 μg) obtained after two rounds of amplification was labeled in a reverse-transcription reaction in the presence of Cy3-labeled (reference sample) or Cy5 (test sample)-labeled dUTP (Amersham, Piscataway, NJ).

  • A widely used protocol, developed by Eberwine and colleagues, uses T7-based linear amplification [ 14 ] . In this procedure, double stranded cDNAs are synthesized from mRNA by reverse-transcription (priming off the 3' polyA tail) and second strand synthesis.

  • Semi-quantitative reverse-transcription PCR (RT-PCR) experiments using exon-specific oligonucleotide primers showed that there were no significant changes in the TAF II 135 or TBP mRNA levels during differentiation (data not shown).

  • Quantitative reverse-transcription polymerase chain reaction

  • eNOS expression in the lung, as studied using quantitative reverse-transcription PCR, was increased 1.5-fold following hypoxia as compared with normoxic conditions, whereas iNOS expression was increased 3.5-fold (Fig.


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