Words similar to reverse-transcribed

Example sentences for: reverse-transcribed

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RNA was extracted from clones and tetramer-positive cells using TRIzol (Invitrogen, Carlsbad, California, United States) and reverse-transcribed into cDNA using SuperScript II Reverse Transcriptase (Invitrogen).
The total RNA samples of HL60 and HL60/TLK286 were reverse-transcribed with Superscript II reverse transcriptase.
For RT-PCR, 1 μg of total RNA was reverse-transcribed into cDNA using SuperscriptII reverse transcriptase (Invitrogen, Carlsbad, CA).
Sorting out of yCCR4-related proteins was performed by i) searches in databases using the yCCR4 C-terminal domain (aa 505-837) conserved in the three previously identified yCCR4-like proteins [ 21 22 ] , and ii) PCR-amplification of cDNA libraries or reverse-transcribed RNAs using appropriate primers followed by sequencing, when required (Table 1).
Briefly, 15 μg of total RNA were reverse-transcribed with an oligo(dT) primer coupled to a T7 RNA polymerase binding site.