Example sentences for: reverse-transcribed

How can you use “reverse-transcribed” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The total RNA samples of HL60 and HL60/TLK286 were reverse-transcribed with Superscript II reverse transcriptase.

  • The cDNA encoding human Rab11 was obtained by PCR amplification from first strand cDNA template, reverse-transcribed from Hela cell mRNA.

  • Total RNA was purified from dissected nephron segments using Tri Reagent (Molecular Research Center) and reverse-transcribed to single-stranded cDNA using Moloney murine leukemia virus reverse transcriptase and 2.5 μM of random hexamers according to the manufacturer's protocol (GeneAmp RNA PCR kit, Perkin Elmer Cetus, Norwalk, CT).

  • For RT-PCR, 1 μg of total RNA was reverse-transcribed into cDNA using SuperscriptII reverse transcriptase (Invitrogen, Carlsbad, CA).

  • Five μg of total RNA was reverse-transcribed according to the manufacturer's protocol (Superscript II Preamplification System, Gibco BRL) using oligo (dT) as primer.


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