Example sentences for: reverse-transcribed

How can you use “reverse-transcribed” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Sorting out of yCCR4-related proteins was performed by i) searches in databases using the yCCR4 C-terminal domain (aa 505-837) conserved in the three previously identified yCCR4-like proteins [ 21 22 ] , and ii) PCR-amplification of cDNA libraries or reverse-transcribed RNAs using appropriate primers followed by sequencing, when required (Table 1).

  • The total RNA samples of HL60 and HL60/TLK286 were reverse-transcribed with Superscript II reverse transcriptase.

  • Briefly, ten μg of total RNA was reverse-transcribed into single stranded cDNA with oligo(dT) 12-18 . Then the double stranded cDNA for HL60 and HL60/TLK286 was generated with the cDNA Synthesis System (Life Technologies, Rockville, MD).

  • For RT-PCR, 1 μg of total RNA was reverse-transcribed into cDNA using SuperscriptII reverse transcriptase (Invitrogen, Carlsbad, CA).

  • The cDNA encoding Rab24 was obtained by PCR amplification from first strand cDNA template reverse-transcribed from mouse brain mRNA, using Taq DNA polymerase (Perkin Elmer).


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