Words similar to reverse-transcribed

Example sentences for: reverse-transcribed

How can you use “reverse-transcribed” in a sentence? Here are some example sentences to help you improve your vocabulary:

For each sample to be analyzed by microarray, 4 μg amplified RNA were primed with 5 μg random hexamers and 5 μg oligo dT and reverse-transcribed, incorporating aa-dUTP, as described at http://www.microarrays.org.
RNA was extracted from clones and tetramer-positive cells using TRIzol (Invitrogen, Carlsbad, California, United States) and reverse-transcribed into cDNA using SuperScript II Reverse Transcriptase (Invitrogen).
Briefly, ten μg of total RNA was reverse-transcribed into single stranded cDNA with oligo(dT) 12-18 . Then the double stranded cDNA for HL60 and HL60/TLK286 was generated with the cDNA Synthesis System (Life Technologies, Rockville, MD).
Five μg of total RNA was reverse-transcribed according to the manufacturer's protocol (Superscript II Preamplification System, Gibco BRL) using oligo (dT) as primer.
Total RNA was purified from dissected nephron segments using Tri Reagent (Molecular Research Center) and reverse-transcribed to single-stranded cDNA using Moloney murine leukemia virus reverse transcriptase and 2.5 μM of random hexamers according to the manufacturer's protocol (GeneAmp RNA PCR kit, Perkin Elmer Cetus, Norwalk, CT).