Example sentences for: reverse-transcribed

How can you use “reverse-transcribed” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Five μg of total RNA was reverse-transcribed according to the manufacturer's protocol (Superscript II Preamplification System, Gibco BRL) using oligo (dT) as primer.

  • RNA was extracted from clones and tetramer-positive cells using TRIzol (Invitrogen, Carlsbad, California, United States) and reverse-transcribed into cDNA using SuperScript II Reverse Transcriptase (Invitrogen).

  • Briefly, ten μg of total RNA was reverse-transcribed into single stranded cDNA with oligo(dT) 12-18 . Then the double stranded cDNA for HL60 and HL60/TLK286 was generated with the cDNA Synthesis System (Life Technologies, Rockville, MD).

  • The cDNA encoding human Rab11 was obtained by PCR amplification from first strand cDNA template, reverse-transcribed from Hela cell mRNA.

  • For each sample to be analyzed by microarray, 4 μg amplified RNA were primed with 5 μg random hexamers and 5 μg oligo dT and reverse-transcribed, incorporating aa-dUTP, as described at http://www.microarrays.org.


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