Words similar to reverse-transcribed

Example sentences for: reverse-transcribed

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For each hybridization, 2 μg of each purified sample of polyadenylated RNA was reverse-transcribed in the presence of fluorescently labeled nucleoside triphosphates (Cy3-dUTP for reference sample, Cy5-dUTP for experimental sample).
The cDNA encoding human Rab11 was obtained by PCR amplification from first strand cDNA template, reverse-transcribed from Hela cell mRNA.
Sorting out of yCCR4-related proteins was performed by i) searches in databases using the yCCR4 C-terminal domain (aa 505-837) conserved in the three previously identified yCCR4-like proteins [ 21 22 ] , and ii) PCR-amplification of cDNA libraries or reverse-transcribed RNAs using appropriate primers followed by sequencing, when required (Table 1).
For each sample to be analyzed by microarray, 4 μg amplified RNA were primed with 5 μg random hexamers and 5 μg oligo dT and reverse-transcribed, incorporating aa-dUTP, as described at http://www.microarrays.org.
Briefly, ten μg of total RNA was reverse-transcribed into single stranded cDNA with oligo(dT) 12-18 . Then the double stranded cDNA for HL60 and HL60/TLK286 was generated with the cDNA Synthesis System (Life Technologies, Rockville, MD).