Example sentences for: reverse-transcribed

How can you use “reverse-transcribed” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The recovered mRNA was reverse-transcribed using AMV-reverse transcriptase (Amersham Pharmacia Biotech Inc., Newark, NJ) and the primer gc3'pET.

  • Five μg of total RNA was reverse-transcribed according to the manufacturer's protocol (Superscript II Preamplification System, Gibco BRL) using oligo (dT) as primer.

  • For RT-PCR, 1 μg of total RNA was reverse-transcribed into cDNA using SuperscriptII reverse transcriptase (Invitrogen, Carlsbad, CA).

  • Briefly, twenty μg of total RNA from HL60 or HL60/TLK286 cells was reverse-transcribed into single stranded cDNA.

  • The cDNA encoding human Rab11 was obtained by PCR amplification from first strand cDNA template, reverse-transcribed from Hela cell mRNA.


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