Words similar to reverse-transcribed
Example sentences for: reverse-transcribed
How can you use “reverse-transcribed” in a sentence? Here are some example sentences to help you improve your vocabulary:
RNA was extracted from clones and tetramer-positive cells using TRIzol (Invitrogen, Carlsbad, California, United States) and reverse-transcribed into cDNA using SuperScript II Reverse Transcriptase (Invitrogen).
For each sample to be analyzed by microarray, 4 μg amplified RNA were primed with 5 μg random hexamers and 5 μg oligo dT and reverse-transcribed, incorporating aa-dUTP, as described at http://www.microarrays.org.
The cDNA encoding Rab24 was obtained by PCR amplification from first strand cDNA template reverse-transcribed from mouse brain mRNA, using Taq DNA polymerase (Perkin Elmer).
Sorting out of yCCR4-related proteins was performed by i) searches in databases using the yCCR4 C-terminal domain (aa 505-837) conserved in the three previously identified yCCR4-like proteins [ 21 22 ] , and ii) PCR-amplification of cDNA libraries or reverse-transcribed RNAs using appropriate primers followed by sequencing, when required (Table 1).
Five μg of total RNA was reverse-transcribed according to the manufacturer's protocol (Superscript II Preamplification System, Gibco BRL) using oligo (dT) as primer.