Example sentences for: reverse-subtraction

How can you use “reverse-subtraction” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The PCR-Select cDNA Subtraction and PCR-Select Differential Screening Kits (Clontech Laboratories Inc.) were used to enrich for and identify differentially expressed genes in the driver and tester cDNAs [ 48 49 ] . Both forward- and reverse-subtraction were performed; untreated HUVEC cDNA served as driver and VEGF-treated HUVEC cDNA served as tester in the former, whereas untreated HUVEC cDNA served as tester and VEGF-treated HUVEC cDNA served as driver in the latter [ 48 49 ] . Target cDNA fragments were amplified using the Marathon™ cDNA Amplification Kit, subcloned into pCR ®2.

  • By employing forward- and reverse-subtraction, we identified both common (indicated by arrows) and differentially expressed genes (Fig.


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