Words similar to replicons
Example sentences for: replicons
How can you use “replicons” in a sentence? Here are some example sentences to help you improve your vocabulary:
Although we knew from previous experiments [ 12 ] that dengue replicons could survive for at least 7 days in culture, the limited durability of cells transfected with gp160-expressing replicons raised the question of whether or not cells transfected with Δpre-M/E-gp120 replicons could survive for similarly long times in culture.
Although our successful development of a plasmid which can express a dengue replicon from transfected DNA facilitates delivery by DNA vaccination [ 12 ] , the development of packaging cell lines which can package these replicons into virions would be a major step forward towards a vaccine which could be conveniently administered in typical clinical situations.
Although we have formally demonstrated expression, not replication, our previous demonstration of the replication [ 12 ] of dengue replicons lacking heterologous material suggests that the replicons described here, which contain heterologous material, are indeed replicating.
Encouraged by the success with GFP, we next looked at Δpre-M/E replicons with HIV-1 env material cloned into the position of the deleted pre-M and E genes.
Previously we have reported the successful construction of several dengue virus replicons which replicate intracellularly without the pre-M and E proteins required to form infectious virions, including one replicon which can be expressed from transfected DNA [ 12 ] . Towards the goal of devising a "live" dual vaccine based on only non-structural dengue proteins and heterologous HIV material, we report here that these replicons can be harnessed to express heterologous genes, including HIV gp160 and gp120.
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