Words similar to replicon
Example sentences for: replicon
How can you use “replicon” in a sentence? Here are some example sentences to help you improve your vocabulary:
The finding of at least one closely apposed pair of cells expressing high levels of replicon proteins on day 9 post transfection, two days after trypsinization and replating (Figure 7, right panel), not only implies replicon replication, but also implies the expression of NS-1 protein as well.
Although this suggests that gp120 expression from a dengue replicon stresses cells, we did find fields with adjacent, gp120 positive cells, suggesting that at least one cell division between day 7 and day 9 had occurred in a cell successfully transfected with Δpre-M/E-gp120 (Figure 7, right panel).
However, when we harvested cultures earlier, at 36 hours post transfection with Δpre-M/E-gp160, intact, fluorescing cells were readily found, though the morphology still appeared atypical compared to either that of cultures transfected with wild type dengue virus and dengue replicons [ 12 ] or the Δpre-M/E-gp120 replicon (see Figure 6).
Demonstration of long term protein expression by a gp120-expressing replicon alone, of course, does not demonstrate that the chimeric dengue replicons constitute an effective vaccine.
Although our successful development of a plasmid which can express a dengue replicon from transfected DNA facilitates delivery by DNA vaccination [ 12 ] , the development of packaging cell lines which can package these replicons into virions would be a major step forward towards a vaccine which could be conveniently administered in typical clinical situations.