Words similar to replicon
Example sentences for: replicon
How can you use “replicon” in a sentence? Here are some example sentences to help you improve your vocabulary:
Expression of genes in the Δpre-M/E-gp120 replicon was reproducibly visualized at 48-50 hours post transfection (Figures 3& 4), at a level of approximatley 1% of the cells, but in many experiments, the corresponding cultures transfected with the gp160 replicon, Δpre-M/E-gp160, either no fluorescence could be visualized (not shown), or only fluorescent cells with a bizarre morphology (characterized by debris and/or degenerative appearance) could be visualized (Figure 5).
The finding of at least one closely apposed pair of cells expressing high levels of replicon proteins on day 9 post transfection, two days after trypsinization and replating (Figure 7, right panel), not only implies replicon replication, but also implies the expression of NS-1 protein as well.
Heterologous genes were cloned into the previously described Δpre-M/E replicon [ 12 ] , into the position previously occupied by the pre-M/E genes.
The frequencies and fluorescence intensities of replicon positive cells seen in the experiments reported here are comparable to those seen for dengue replicons lacking heterologous material [ 12 ] , suggesting that replication of the Δpre-M/E replicons containing heterologous material is occurring as well.
Although our successful development of a plasmid which can express a dengue replicon from transfected DNA facilitates delivery by DNA vaccination [ 12 ] , the development of packaging cell lines which can package these replicons into virions would be a major step forward towards a vaccine which could be conveniently administered in typical clinical situations.
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