Words similar to replicon

Example sentences for: replicon

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Expression of genes in the Δpre-M/E-gp120 replicon was reproducibly visualized at 48-50 hours post transfection (Figures 3& 4), at a level of approximatley 1% of the cells, but in many experiments, the corresponding cultures transfected with the gp160 replicon, Δpre-M/E-gp160, either no fluorescence could be visualized (not shown), or only fluorescent cells with a bizarre morphology (characterized by debris and/or degenerative appearance) could be visualized (Figure 5).
Demonstration of long term protein expression by a gp120-expressing replicon alone, of course, does not demonstrate that the chimeric dengue replicons constitute an effective vaccine.
Although this suggests that gp120 expression from a dengue replicon stresses cells, we did find fields with adjacent, gp120 positive cells, suggesting that at least one cell division between day 7 and day 9 had occurred in a cell successfully transfected with Δpre-M/E-gp120 (Figure 7, right panel).
Even the gp120-expressing replicon seems mildly toxic in that the frequency of gp120 positive cells declines with time in culture post transfection with Δpre-M/E-gp120 (not shown).
The full length virus and replicon cDNA plasmids isolated from STBL 2 cells were linearized with Sac I, purified by Qiagen chromatography, and eluted by RNAase-free water in preparation for transcription.