Words similar to replicon
Example sentences for: replicon
How can you use “replicon” in a sentence? Here are some example sentences to help you improve your vocabulary:
Expression of genes in the Δpre-M/E-gp120 replicon was reproducibly visualized at 48-50 hours post transfection (Figures 3& 4), at a level of approximatley 1% of the cells, but in many experiments, the corresponding cultures transfected with the gp160 replicon, Δpre-M/E-gp160, either no fluorescence could be visualized (not shown), or only fluorescent cells with a bizarre morphology (characterized by debris and/or degenerative appearance) could be visualized (Figure 5).
The finding of at least one closely apposed pair of cells expressing high levels of replicon proteins on day 9 post transfection, two days after trypsinization and replating (Figure 7, right panel), not only implies replicon replication, but also implies the expression of NS-1 protein as well.
Although this suggests that gp120 expression from a dengue replicon stresses cells, we did find fields with adjacent, gp120 positive cells, suggesting that at least one cell division between day 7 and day 9 had occurred in a cell successfully transfected with Δpre-M/E-gp120 (Figure 7, right panel).
Demonstration of long term protein expression by a gp120-expressing replicon alone, of course, does not demonstrate that the chimeric dengue replicons constitute an effective vaccine.
The full length virus and replicon cDNA plasmids isolated from STBL 2 cells were linearized with Sac I, purified by Qiagen chromatography, and eluted by RNAase-free water in preparation for transcription.
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