Words similar to replicon
Example sentences for: replicon
How can you use “replicon” in a sentence? Here are some example sentences to help you improve your vocabulary:
Heterologous genes were cloned into the previously described Δpre-M/E replicon [ 12 ] , into the position previously occupied by the pre-M/E genes.
Even the gp120-expressing replicon seems mildly toxic in that the frequency of gp120 positive cells declines with time in culture post transfection with Δpre-M/E-gp120 (not shown).
However, when we harvested cultures earlier, at 36 hours post transfection with Δpre-M/E-gp160, intact, fluorescing cells were readily found, though the morphology still appeared atypical compared to either that of cultures transfected with wild type dengue virus and dengue replicons [ 12 ] or the Δpre-M/E-gp120 replicon (see Figure 6).
The finding of at least one closely apposed pair of cells expressing high levels of replicon proteins on day 9 post transfection, two days after trypsinization and replating (Figure 7, right panel), not only implies replicon replication, but also implies the expression of NS-1 protein as well.
The frequencies and fluorescence intensities of replicon positive cells seen in the experiments reported here are comparable to those seen for dengue replicons lacking heterologous material [ 12 ] , suggesting that replication of the Δpre-M/E replicons containing heterologous material is occurring as well.
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