Words similar to recombinase
Example sentences for: recombinase
How can you use “recombinase” in a sentence? Here are some example sentences to help you improve your vocabulary:
We have constructed two reporter lines of mice that express EYFP or ECFP only in cells expressing the Cre recombinase, and their daughter cells, by targeting these cDNAs into the ubiquitously expressed ROSA26 locus, preceded by a loxP flanked "stop" sequence.
More recently, transgenic mice have also been used to express Cre recombinase to mediate the conditional deletion of loxP -flanked DNA sequences in specific cell types in vivo . Traditional methods for generating transgenic mice require the availability of well characterized transcriptional regulatory elements, promoters or enhancers, that direct transgene expression in specific cell types.
An example in the case of the recombinase genes is the loss of recF, which is required for some recA functions [ 38] and which was deleted individually with both flanking genes retained (Table 1).
The pgk promoter was flanked by loxP elements so that it could be deleted by the action of Cre recombinase.
Interestingly, the positions exhibiting the strongest correlation overlap substantially with the RSS nucleotides that contact the recombinase (Figure 2and [ 53]), offering support for the hypothesis that positions acting cooperatively to influence binding by the recombinase coevolve.