Words similar to recombinant
Example sentences for: recombinant
How can you use “recombinant” in a sentence? Here are some example sentences to help you improve your vocabulary:
Rabbit antibodies against peptides from HIV-1 IIIB gp120/gp41 and against the V3 loop of HIV-1 BaL (anti-V3 BaL) were prepared as described [ 16 ] . Antiserum to phthalate was prepared by immunization of rabbits with phthalic anhydride treated rabbit serum albumin [ 17 ] . Recombinant soluble CD4 (sCD4) was from Genentech Inc., South San Francisco, CA.
To identify components with PEDF-binding affinity, purified recombinant protein was used to prepare PEDF-affinity resin with 3 M Emphaze™ Biosupport Medium (Pierce Chemical) [ 18 ] . CM awas dialyzed against buffer P (20 mM sodium phosphate pH 7, 150 mM NaCl, 0.5% CHAPS) and filtrated through 0.4 μm filters.
A cDNA that encodes a hemagglutinin-tagged NPM3 protein was produced by PCR under the following conditions: 100 μl reactions with 1X Pfu buffer, 0.2 mM deoxyribonucleotides, 1 μM HA-F primer (5'-AAA GAA TTC AGC ATG TAC CCA TAC GAC GTC CCA GAC TAC GCC GCC GCC GGT ACT GCA GCT GCC-3'), 1 μM NPM3 -R2 primer (5'-AAA GAA TTC CTA GGG CCT GCC CCC CTG CTT TTT GGC AGG AAG GAT GGG-3'), 1 ng of human NPM3 cDNA insert, and 2.5 Units of recombinant Pfu DNA Polymerase.
Following isolation of pure plaques, the insert DNAs were prepared by PCR methods with the following conditions: 100 μl reactions containing 1X Pfu buffer (20 mM Tris-Cl, pH 8.75, 10 mM KCl, 2 mM MgSO 4 , 10 mM (NH 4 ) 2 SO 4 , 0.1% v/v Triton X-100, 100 μg/ml bovine serum albumin), 1 μM λgt11 forward and reverse primers (Promega, Madison, WI), 0.2 mM deoxyribonucleotides, and 5 Units of recombinant Pfu DNA Polymerase (Stratagene, La Jolla, CA).
Notably, whereas the four HSV-2 parental viruses had spontaneous mutation frequencies significantly higher than HSV-1 isolates (Table 1), the frequency of errors from pol -recombinant viruses expressing an HSV-2 Pol in an HSV-1 background were indistinguishable from HSV-1 SC16 when tested in MRC-5 cells.
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