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Example sentences for: recbc
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The accumulation of the non-circular, broken chromosomes correlated with the growth rate or DNA damage response in most of the recBC -minus background [ 30 ] . The recB or recC null mutation showed low viability even in the absence of exogenous DNA damage [ 31 32 ] . A simple interpretation of these data is that RecA, RecFOR, and RecQJ functions (and RecET functions for the sbcA background) repair chromosome breakage and/or prevent generation of the breakage.
In the recBC sbcBC strain, RecBCD enzyme is inactive and RecFOR and RecQJ proteins promote recombination together with RecA [ 19 ] . In the recBC sbcBC background, recA, recF, recJ, recQ and recR mutants accumulated these huge linears to varying extents (Figure 2D).
Game and his colleagues have developed a sensitive means of detecting chromosomal ds breakage using a circular chromosome [ 3 ] . Under most conditions of pulsed-field gel electrophoresis, a circular yeast chromosome and circular bacterial chromosomes will not enter the gel, very likely because they are trapped by the branches of the network of agarose [ 3 13 14 ] . One double-strand break transforms this circular form into a linear form, which can now move slowly in the gel [ 3 ] . We used this procedure to detect double-strand breakage of a circular bacterial chromosome occurring spontaneously or after loss of a restriction-modification gene complex [ 15 16 ] . We found increased chromosome breakage in recBC -null and recC1002 mutants of E. coli under both conditions [ 1 ] . Michel and her colleagues used pulsed-field gel electrophoresis to detect degraded chromosomal DNAs arising spontaneously in recBC mutants and arising during replication fork arrest [ 2 ] . RuvABC proteins, which catalyze migration and cleavage of Holliday junctions, are responsible for the occurrence of the degraded DNAs following replication fork arrests [ 17 ] .
The accumulation of huge linear DNAs was also seen in other recBC null mutants in this AB1157 genetic background ( recB21 , recC22 and recC73 (Figure 2A)) and in another, V66, genetic background ( recC73 (Figure 2B)), as observed earlier [ 1 2 ] . A recD mutant showed no accumulation.
The accumulation by the recJ mutation in the recBC sbcA background is suppressed by a recQ mutation (Figure 2C).
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