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Example sentences for: recbc
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In the recBC sbcA strain, an sbcA mutation on the Rac prophage expresses recET genes, which promotes homologous recombination at a ds end [ 7 ] . The accumulation of the huge linears was seen with recA, recE, recT, recJ, recQ, recF, recO and recR strains (Figure 2C).
The accumulation of huge linear DNAs was also seen in other recBC null mutants in this AB1157 genetic background ( recB21 , recC22 and recC73 (Figure 2A)) and in another, V66, genetic background ( recC73 (Figure 2B)), as observed earlier [ 1 2 ] . A recD mutant showed no accumulation.
In the recBC sbcA and the recBC sbcBC backgrounds, the absence of RecBCD nuclease may prevent faster degradation of the large non-circular DNAs.
Partial suppression of the accumulation of the huge linears in a recBC null allele by a recF mutation (Figure 2B) leads to several possible explanations.
Game and his colleagues have developed a sensitive means of detecting chromosomal ds breakage using a circular chromosome [ 3 ] . Under most conditions of pulsed-field gel electrophoresis, a circular yeast chromosome and circular bacterial chromosomes will not enter the gel, very likely because they are trapped by the branches of the network of agarose [ 3 13 14 ] . One double-strand break transforms this circular form into a linear form, which can now move slowly in the gel [ 3 ] . We used this procedure to detect double-strand breakage of a circular bacterial chromosome occurring spontaneously or after loss of a restriction-modification gene complex [ 15 16 ] . We found increased chromosome breakage in recBC -null and recC1002 mutants of E. coli under both conditions [ 1 ] . Michel and her colleagues used pulsed-field gel electrophoresis to detect degraded chromosomal DNAs arising spontaneously in recBC mutants and arising during replication fork arrest [ 2 ] . RuvABC proteins, which catalyze migration and cleavage of Holliday junctions, are responsible for the occurrence of the degraded DNAs following replication fork arrests [ 17 ] .
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