Words similar to reca
Example sentences for: reca
How can you use “reca” in a sentence? Here are some example sentences to help you improve your vocabulary:
In the recBC sbcBC strain, RecBCD enzyme is inactive and RecFOR and RecQJ proteins promote recombination together with RecA [ 19 ] . In the recBC sbcBC background, recA, recF, recJ, recQ and recR mutants accumulated these huge linears to varying extents (Figure 2D).
An example in the case of the recombinase genes is the loss of recF, which is required for some recA functions [ 38] and which was deleted individually with both flanking genes retained (Table 1).
Studies in Escherichia coli have shown that, although RecA is the principle protein involved in pairing and strand exchange, unrelated proteins, that have a much more restrictive phyletic distribution, can also promote similar reactions in a RecA dependent or RecA-independent manner [ 3 ] . These alternative or additional mediators of homologous recombination include the well-characterized prophage RecT, phage λ Redβ and phage P22 ERF proteins [ 4 5 ] . Similarly, in yeast and vertebrates, the RAD52 protein is involved in the pairing and strand exchange reaction and can promote recombination in a RAD51 (the eukaryotic RecA homolog)-dependent or independent manner [ 6 ] . The RecT protein works in conjunction with the RecE-nuclease [ 7 ] and was initially described in genetic studies on the complemention of mutations in the RecBCD pathway of DNA repair [ 8 9 10 ] . Biochemically, RecT has been shown to bind single-stranded (ss) DNA 3' overhang regions generated by the RecE nuclease, and promote strand exchange between homologous DNA partners by assisting the pairing of complementary single-stranded regions [ 4 10 ] . The reaction catalyzed by the RecT/RecE system is similar to that described for the phage λ exonuclease (exo/Redβ) and the single-strand annealing protein Redβ.
In the recBC sbcA strain, an sbcA mutation on the Rac prophage expresses recET genes, which promotes homologous recombination at a ds end [ 7 ] . The accumulation of the huge linears was seen with recA, recE, recT, recJ, recQ, recF, recO and recR strains (Figure 2C).
The accumulation of the non-circular, broken chromosomes correlated with the growth rate or DNA damage response in most of the recBC -minus background [ 30 ] . The recB or recC null mutation showed low viability even in the absence of exogenous DNA damage [ 31 32 ] . A simple interpretation of these data is that RecA, RecFOR, and RecQJ functions (and RecET functions for the sbcA background) repair chromosome breakage and/or prevent generation of the breakage.
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