Words similar to rec
Example sentences for: rec
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At present, two hypothetical mechanisms by which a Rec12-dependent signal affects MII are proposed: First, Rec12-dependent (recombination-dependent) signals might trigger a block after MI, thereby allowing cells to skip a frequently irregular MII division [ 39 ] . However, the majority of the rec12 mutant meioses show evidence of two meiotic divisions (Figure 3) and many of the meiotic diploids exhibit mixed MI and MII missegregation patterns for centromere linked markers on the three different chromosomes (Table 2).
While there was clear cytological evidence of two meiotic divisions and aberrant chromosome assortment in each division in the majority of rec12 mutant cells (Figure 3), it remained possible that some of the diploid meiotic products were derived from cells that had skipped one of the two meiotic divisions, thereby biasing the chromosome segregation data.
Identification of four introns (Figure 1) and complete complementation of the rec12-117 hyporecombination defect by the full-length cDNA (Figure 2) confirmed that Rec12 protein is homologous to Spo11 of other eukaryotes [ 11 ] . Both Rec12 and its active site tyrosine are required for intragenic and intergenic recombination at multiple locations on all three chromosomes (Figure 2, Tables 1, 2, data not shown).
Very low levels of ectopic rec12 +expression restored wild-type recombination levels to the rec12-117 mutant (Figure 2B), confirming that functional Rec12 protein was produced.
Rec12 has a role in equational chromosome segregation during Meiosis II that is dependent upon its active site tyrosine
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