Words similar to rb-
Example sentences for: rb-
How can you use “rb-” in a sentence? Here are some example sentences to help you improve your vocabulary:
The immune complex was centrifuged, and the proteins in the immune complex were resolved by 10% SDS-PAGE followed by western blot analysis using anti-pRb-2/p130 antibodies as already described for western blot analysis.
Monoclonal antibodies used in the study were: 1) mAb against human HLA-DR,DP,DQ, FITC conjugate (anti-MHCII-FITC), clone Tü39, mouse IgG2a; 2) mAb against human IFNg receptor R1 chain, biotin conjugate, clone MMHGR-1, mouse IgG1; 3) mAb against human IFNg receptor R2 chain, biotin conjugate, clone MMHGR-2, mouse IgG1; 4) mouse IgG2a isotype control mAb, FITC conjugate (IgG2a-FITC); 5) mouse IgG1 isotype control mAb, biotin conjugate; 6) mAb against Rb protein, FITC conjugate (Rb-FITC), clone G3-245, mouse IgG1; 7) mouse IgG1 isotype control mAb, FITC conjugate (IgG1-FITC).
The same sequential steps were followed for pRb-2/p130 immunostaining using anti-pRb-2/p130 monoclonal antibodies (Santa Cruz Biotechnology).
Antibodies: SKP2 (Zymed 32-3300, 1:250 for immunoblotting), SKP2 (Santa Cruz sc-7164, 1:500 for immunofluorescence staining), p27 (Transduction Laboratories K25030, 1:5000 for immunoblotting, 1:1000 for immunofluorescence staining), cyclin A (Neomarkers Ab-6, MS-1061, 1:100 for immunoblotting), cyclin E (Neomarkers Ab-1, RB-012, 2 ug per immunoprecipitation) cyclin E (Neomarkers Ab-2, MS-870, 1:200 for immunoblotting), HRT1 (affinity-purified rabbit polyclonal, gift from R. Deshaies, 1:1000 for immunoblotting), CUL1 (Zymed 71-8700, 1:250 for immunoblotting), SKP1 (Transduction laboratories 610530, 1:5000 for immunoblotting), JAB1/CSN5 (GeneTex MS-Jab11-PX1, 1:1000 for immunoblotting), RB1 (Pharmingen 554136, 1:500 for immunoblotting), CDK2 (Santo Cruz Biotechnology sc-163, 2 ug per immunoprecipitation, 1:500 for immunoblotting), Tubulin (Sigma T5168, 1:2000 for immunoblotting).
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