Words similar to ratpase

Example sentences for: ratpase

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The ligation products were amplified with an adapter-specific primer and either the probe-specific forward primer RATPase6F or SP-BF (for the T7 adapter ligation) or the probe-specific reverse primer RATPase6R or SP-BR (for the SP6 adapter ligation).
Four PCR products were thus generated: RATPase6F-T7 adapter, SP6 adapter-RATPase6R, SP-BF-T7 adapter, and SP6 adapter-SP-BR.
The ATPase 6 clones were used as templates in PCRs with primer pairs RATPase6F (5'-ACCCCCATCTCACTAATTCC-3') and RATPase6R (5'-AGTACTAGGGTAGCTCCTCC-3') to generate a 145 bp fragment to make ATPase 6 riboprobes.
The RATPase6F-T7 adapter and SP-BF-T7 adapter PCR products were used as templates for in vitro transcription to produce antisense riboprobes, and the SP6 adapter-RATPase6R or SP6 adapter-SP-BR PCR PCR products were used to make sense probes.