Words similar to radiolabelled

Example sentences for: radiolabelled

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Control tubes contained 1000-fold excess of the corresponding unlabeled recombinant human chemokine (molecular mass as for radiolabelled chemokines) (PeproTech EC, London, UK; CXCL8 was kindly donated from Novartis Research Institute, Vienna, Austria).
Our results agree with in situ studies in human skin, where macrophages also express binding sites for radiolabelled CCL3, CCL5 and CXCL8 [ 24 25 ] . The disadvantage with the radioligand technique is the lack of definition regarding the molecular nature of the receptors.
To look for the role of monoclonal antibodies against IROMPs in blocking the iron uptake the bacterial cells were pre-incubated with 100 μl of undiluted monoclonal antibody culture supernatant for 1 h at 37°C before addition of radiolabelled iron siderophore complex.
In vitro translated (IVT) proteins were produced and radiolabelled with 35S-methionine using the TNT coupled wheat germ extract system from Promega.
At the end the unbound radiolabelled iron was removed by chromatography using sephadex G-10 as described by Rosenberg [ 44 ] . The uptake of radiolabelled iron siderophore was studied by the method described by Rosenberg [ 44 ] . Briefly A. baumannii was grown in CDM-Fe medium also in CDM+Fe medium to a mid exponential phase and was washed twice with uptake medium (30 mM Na 2 HPO 4 , 10 mM K 2 HPO 4 , 10 mM (NH 4 ) 2 SO 4 ,1 mM MgCl 2 and 40 μM CaCl 2 pH 6.9) at 4°C and re-suspended in the same medium at a density of A 470 = 0.4.